التفاصيل البيبلوغرافية
| العنوان: |
Pituitary adenylate cyclase-activating polypeptide plays a key role in nitroglycerol-induced trigeminovascular activation in mice |
| المؤلفون: |
Adrienn Markovics, Viktoria Kormos, Balazs Gaszner, Arvin Lashgarara, Eva Szoke, Katalin Sandor, Krisztina Szabadfi, Bernadett Tuka, Janos Tajti, Janos Szolcsanyi, Erika Pinter, Hitoshi Hashimoto, Jozsef Kun, Dora Reglodi, Zsuzsanna Helyes |
| المصدر: |
Neurobiology of Disease, Vol 45, Iss 1, Pp 633-644 (2012) |
| بيانات النشر: |
Elsevier, 2012. |
| سنة النشر: |
2012 |
| المجموعة: |
LCC:Neurosciences. Biological psychiatry. Neuropsychiatry |
| مصطلحات موضوعية: |
Light-aversive behavior, Light–dark box, Laser Doppler blood perfusion scanning, Meningeal microcirculation, c-Fos immunohistochemistry, PAC1 receptor immunohistochemistry, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571 |
| الوصف: |
Pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptors (PAC1, VPAC) are present in sensory neurons and vascular smooth muscle. PACAP infusion was found to trigger migraine-like headache in humans and we showed its central pro-nociceptive function in several mouse pain models. Nitroglycerol (NTG)-induced pathophysiological changes were investigated in this study in PACAP gene-deleted (PACAP−/−) and wildtype (PACAP+/+) mice. Chemical activation of the trigeminovascular system was induced by 10 mg/kg i.p. NTG. Light-aversive behavior was determined in a light–dark box, meningeal microcirculation by laser Doppler blood perfusion scanning and the early neuronal activation marker c-Fos with immunohistochemistry. NTG-induced photophobia both in the early (0–30 min) and late phases (90–120 min) due to direct vasodilation and trigeminal sensitization, respectively, was significantly reduced in PACAP−/− mice. Meningeal blood flow increased by 30–35% during 4 h in PACAP+/+ mice, but only a 5–10% elevation occurred from the second hour in PACAP−/− ones. The number of c-Fos expressing cells referring to neuronal activation in the trigeminal ganglia and nucleus caudalis significantly increased 4 h after NTG in PACAP+/+, but not in PACAP−/− animals. Similar PAC1 receptor immunostaining was detected in both groups, which did not change 4 h after NTG treatment. PACAP-38 (300 μg/kg, i.p.) produced photophobia similarly to NTG and 30% meningeal vasodilatation for 30 min in PACAP+/+, but not in PACAP−/− mice. It significantly increased neural activation 4 h later in the trigeminal ganglia of both groups, but in the nucleus caudalis of only the PACAP+/+ mice.We provide the first experimental results that PACAP is a pivotal mediator of trigeminovascular activation/sensitization and meningeal vasodilation related to migraine. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1095-953X |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S0969996111003421; https://doaj.org/toc/1095-953X |
| DOI: |
10.1016/j.nbd.2011.10.010 |
| URL الوصول: |
https://doaj.org/article/af168d60755641929d761c3edfb1b7ff |
| رقم الانضمام: |
edsdoj.f168d60755641929d761c3edfb1b7ff |
| قاعدة البيانات: |
Directory of Open Access Journals |