Academic Journal
أعرض في EDS

Real-time PCR for rapid in planta detection of Plectosphaerella cucumerina on wild rocket (Diplotaxis tenuifolia)

التفاصيل البيبلوغرافية
العنوان: Real-time PCR for rapid in planta detection of Plectosphaerella cucumerina on wild rocket (Diplotaxis tenuifolia)
المؤلفون: Giovanna GILARDI, Kathryn WEBB, Giuseppe ORTU, Maria Lodovica GULLINO, Angelo GARIBALDI
المصدر: Phytopathologia Mediterranea, Vol 55, Iss 2 (2016)
بيانات النشر: Firenze University Press, 2016.
سنة النشر: 2016
المجموعة: LCC:Botany
مصطلحات موضوعية: diagnosis, leafy vegetables, Botany, QK1-989
الوصف: Plectosphaerella cucumerina that has recently been described as causing a leaf spot on wild rocket (Diplotaxis tenuifolia) in Italy, but has not yet been recorded on this host in other countries. This fungus has the potential to cause severe economic losses to the salad-producing industry, especially in Italy. This paper describes the development of a real-time PCR assay based on internal transcribed spacer (ITS) sequence, to aid diagnosis of this pathogen in culture and in plant material. The assay showed no cross reaction with closely related Plectosphaerella species or with 18 other commonly found fungi, and was able to detect 10 fg of Pa. cucumerina DNA per reaction. The assay was more specific than previously available molecular methods. A comparison of molecular and isolation methods indicated that the real-time PCR method is suitable for detection of the pathogen on plant material.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 0031-9465
1593-2095
Relation: https://oajournals.fupress.net/index.php/pm/article/view/5668; https://doaj.org/toc/0031-9465; https://doaj.org/toc/1593-2095
DOI: 10.14601/Phytopathol_Mediterr-15108
URL الوصول: https://doaj.org/article/f071890e188b4ef8b1c2306c41e0e4f0
رقم الانضمام: edsdoj.f071890e188b4ef8b1c2306c41e0e4f0
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:00319465
15932095
DOI:10.14601/Phytopathol_Mediterr-15108