التفاصيل البيبلوغرافية
| العنوان: |
Comparing Different Methods for the Diagnosis of Liver Steatosis: What Are the Best Diagnostic Tools? |
| المؤلفون: |
Sophie Chopinet, Olivier Lopez, Sophie Brustlein, Antoine Uzel, Anais Moyon, Isabelle Varlet, Laure Balasse, Frank Kober, Mickaël Bobot, Monique Bernard, Aurélie Haffner, Michaël Sdika, Bruno Montcel, Benjamin Guillet, Vincent Vidal, Emilie Grégoire, Jean Hardwigsen, Pauline Brige |
| المصدر: |
Diagnostics, Vol 14, Iss 20, p 2292 (2024) |
| بيانات النشر: |
MDPI AG, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Medicine (General) |
| مصطلحات موضوعية: |
liver steatosis, MCD diet, CARS microscopy, MR spectroscopy, 99mTc MIBI, near-infrared spectroscopy, Medicine (General), R5-920 |
| الوصف: |
Background: Due to the ongoing organ shortage, marginal grafts with steatosis are more frequently used in liver transplantation, leading to higher occurrences of graft dysfunction. A histological analysis is the gold standard for the quantification of liver steatosis (LS), but has its drawbacks: it is an invasive method that varies from one pathologist to another and is not available in every hospital at the time of organ procurement. This study aimed to compare non-invasive diagnostic tools to a histological analysis for the quantification of liver steatosis. Methods: Male C57BL6J mice were fed with a methioninecholine-deficient (MCD) diet for 14 days or 28 days to induce LS, and were compared to a control group of animals fed with a normal diet. The following non-invasive techniques were performed and compared to the histological quantification of liver steatosis: magnetic resonance spectroscopy (MRS), CARS microscopy, 99mTc MIBI SPECT imaging, and a new near-infrared spectrometer (NIR-SG1). Results: After 28 days on the MCD diet, an evaluation of LS showed ≥30% macrovesicular steatosis. High correlations were found between the NIR-SG1 and the blinded pathologist analysis (R2 = 0.945) (p = 0.001), and between the CARS microscopy (R2 = 0.801 (p < 0.001); MRS, R2 = 0.898 (p < 0.001)) and the blinded pathologist analysis. The ROC curve analysis showed that the area under the curve (AUC) was 1 for both the NIR-SG1 and MRS (p = 0.021 and p < 0.001, respectively), while the AUC = 0.910 for the Oil Red O stain (p < 0.001) and the AUC = 0.865 for the CARS microscopy (p < 0.001). The AUC for the 99mTc MIBI SPECT was 0.640 (p = 0.013), and this was a less discriminating technique for LS quantification. Conclusions: The best-performing non-invasive methods for LS quantification are MRS, CARS microscopy, and the NIR-SG1. The NIR-SG1 is particularly appropriate for clinical practice and needs to be validated by clinical studies on liver grafts. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2075-4418 |
| Relation: |
https://www.mdpi.com/2075-4418/14/20/2292; https://doaj.org/toc/2075-4418 |
| DOI: |
10.3390/diagnostics14202292 |
| URL الوصول: |
https://doaj.org/article/bf9f55c162504688b964a47ccd68ff97 |
| رقم الانضمام: |
edsdoj.bf9f55c162504688b964a47ccd68ff97 |
| قاعدة البيانات: |
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