Academic Journal
Mild Hypothermia Inhibits Differentiation of Human Embryonic and Induced Pluripotent Stem Cells
| العنوان: | Mild Hypothermia Inhibits Differentiation of Human Embryonic and Induced Pluripotent Stem Cells |
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| المؤلفون: | Glenn S. Belinsky, Srdjan D. Antic |
| المصدر: | BioTechniques, Vol 55, Iss 2, Pp 79-82 (2013) |
| بيانات النشر: | Taylor & Francis Group, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | temperature, culture, unwanted differentiation, spontaneous differentiation, cold-inducible gene, Biology (General), QH301-705.5 |
| الوصف: | Culture of pluripotent stem cells at 35°C strikingly reduces unwanted spontaneous differentiation during hESC and iPSC maintenance compared with 37°C. Growth at 35°C did not affect expression of pluripotency mRNAs nor induce expression of cold-inducible genes. Colony size was somewhat reduced at 35°C. Thus, growth at 35°C is a convenient, simple method to reduce the labor of removing spontaneously differentiated colonies when maintaining pluripotent cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1940-9818 0736-6205 |
| Relation: | https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818 |
| DOI: | 10.2144/000114065 |
| URL الوصول: | https://doaj.org/article/eebe649c81c34106b22575559ebcc38e |
| رقم الانضمام: | edsdoj.be649c81c34106b22575559ebcc38e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19409818 07366205 |
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| DOI: | 10.2144/000114065 |