التفاصيل البيبلوغرافية
| العنوان: |
Differential Gene Expression Pattern of Importin β3 and NS5 in C6/36 Cells Acutely and Persistently Infected with Dengue Virus 2 |
| المؤلفون: |
María Leticia Ávila-Ramírez, Ana Laura Reyes-Reyes, Rodolfo Gamaliel Avila-Bonilla, Mariana Salas-Benito, Doris Cerecedo, María Esther Ramírez-Moreno, María Elena Villagrán-Herrera, Ricardo Francisco Mercado-Curiel, Juan Santiago Salas-Benito |
| المصدر: |
Pathogens, Vol 12, Iss 2, p 191 (2023) |
| بيانات النشر: |
MDPI AG, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Medicine |
| مصطلحات موضوعية: |
dengue virus, viral persistence, importin, nuclear transport, Medicine |
| الوصف: |
The establishment of persistent dengue virus infection within the cells of the mosquito vector is an essential requirement for viral transmission to a new human host. The mechanisms involved in the establishment and maintenance of persistent infection are not well understood, but it has been suggested that both viral and cellular factors might play an important role. In the present work, we evaluated differential gene expression in Aedes albopictus cells acutely (C6/36-HT) and persistently infected (C6-L) with Dengue virus 2 by cDNA-AFLP. We observed that importin β3 was upregulated in noninfected cells compared with C6-L cells. Using RT-qPCR and plaque assays, we observed that Dengue virus levels in C6-L cells essentially do not vary over time, and peak viral titers in acutely infected cells are observed at 72 and 120 h postinfection. The expression level of importin β3 was higher in acutely infected cells than in persistently infected cells; this correlates with higher levels of NS5 in the nucleus of the cell. The differential pattern of importin β3 expression between acute and persistent infection with Dengue virus 2 could be a mechanism to maintain viral infection over time, reducing the antiviral response of the cell and the viral replicative rate. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2076-0817 |
| Relation: |
https://www.mdpi.com/2076-0817/12/2/191; https://doaj.org/toc/2076-0817 |
| DOI: |
10.3390/pathogens12020191 |
| URL الوصول: |
https://doaj.org/article/bd5a32e0cfe34ca2b621d92e58c2db9e |
| رقم الانضمام: |
edsdoj.bd5a32e0cfe34ca2b621d92e58c2db9e |
| قاعدة البيانات: |
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