Academic Journal
The metatranscriptomes of root caries and sound root surface biofilms
| العنوان: | The metatranscriptomes of root caries and sound root surface biofilms |
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| المؤلفون: | Thuy Do, Naile Dame-Teixeira, Clarissa C.F. Parolo, Marisa Maltz, Aradhna Tugnait, Deirdre Devine |
| المصدر: | Journal of Oral Microbiology, Vol 9, Iss 0 (2017) |
| بيانات النشر: | Taylor & Francis Group, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | There is limited knowledge of bacterial metabolism in root caries lesions. The aim of this study was to describe the bacterial metatranscriptomes associated with root caries and sound root surfaces using an RNA-seq analysis approach. The biofilms from exposed root surfaces were sampled from caries-free volunteers (n=10), and from the infected dentine of volunteers with root caries (n=30). Total bacterial RNA was extracted; cDNA libraries were prepared and sequenced on the Illumina Hi-Seq2500. The function and composition of the metabolically active microbiota were investigated using: a) MG-RAST, and b) denovo assembly of the read data and mapping to contigs. Differential gene expression analysis was done using the R package DESeq2 (padj |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2000-2297 20002297 |
| Relation: | https://doaj.org/toc/2000-2297 |
| DOI: | 10.1080/20002297.2017.1325195 |
| URL الوصول: | https://doaj.org/article/ebd58a83b8ac4a4591665d3940cc8b81 |
| رقم الانضمام: | edsdoj.bd58a83b8ac4a4591665d3940cc8b81 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20002297 |
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| DOI: | 10.1080/20002297.2017.1325195 |