التفاصيل البيبلوغرافية
| العنوان: |
Ileal proteomic changes associated with IL-25-mediated resistance against intestinal trematode infections |
| المؤلفون: |
María Álvarez-Izquierdo, J. Guillermo Esteban, Carla Muñoz-Antoli, Rafael Toledo |
| المصدر: |
Parasites & Vectors, Vol 13, Iss 1, Pp 1-17 (2020) |
| بيانات النشر: |
BMC, 2020. |
| سنة النشر: |
2020 |
| المجموعة: |
LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: |
Proteomics, Intestine, Interleukin-25, Intestinal helminths, Trematoda, Echinostoma caproni, Infectious and parasitic diseases, RC109-216 |
| الوصف: |
Abstract Background Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode, which has been extensively used to investigate the factors that determine the rejection of intestinal helminths. In this sense, several studies have shown that IL-25 is critical for the development of resistance against E. caproni in mice. In fact, treatment of mice with recombinant IL-25 generates resistance against primary E. caproni infection. However, the mechanisms by which IL-25 induces resistance remain unknown. Methods To study the mechanisms responsible for resistance elicited by IL-25, we analyzed the ileal proteomic changes induced by IL-25 in mice and their potential role in resistance. To this purpose, we compared the protein expression profiles in the ileum of four experimental groups of mice: naïve controls; E. caproni-infected mice; rIL-25-treated mice; and rIL-25-treated mice exposed to E. caproni metacercariae. Results Quantitative comparison by 2D-DIGE showed significant changes in a total of 41 spots. Of these, 40 validated protein spots were identified by mass spectrometry corresponding to 24 proteins. Conclusions Our results indicate that resistance to infection is associated with the maintenance of the intestinal epithelial homeostasis and the regulation of proliferation and cell death. These results provide new insights into the proteins involved in the regulation of tissue homeostasis after intestinal infection and its transcendence in resistance. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1756-3305 |
| Relation: |
http://link.springer.com/article/10.1186/s13071-020-04206-y; https://doaj.org/toc/1756-3305 |
| DOI: |
10.1186/s13071-020-04206-y |
| URL الوصول: |
https://doaj.org/article/bd41f9fc4a0b4874818c982d439b1cc3 |
| رقم الانضمام: |
edsdoj.bd41f9fc4a0b4874818c982d439b1cc3 |
| قاعدة البيانات: |
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