التفاصيل البيبلوغرافية
| العنوان: |
Using inducible lentiviral vectors to generate induced pluripotent stem cell line ZOCi001-A from peripheral blood cells of a patient with CRB1−/− retinitis pigmentosa. |
| المؤلفون: |
Xiangcheng Tang, Xinyu Liu, Zhigang Chen, Lixia Luo, Xialin Liu, Juan Deng, David Wan-Cheng Li, Yizhi Liu |
| المصدر: |
Stem Cell Research, Vol 45, Iss , Pp - (2020) |
| بيانات النشر: |
Elsevier, 2020. |
| سنة النشر: |
2020 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
Biology (General), QH301-705.5 |
| الوصف: |
We isolated peripheral blood mononuclear cells from a patient diagnosed with early-onset non-syndromic retinitis pigmentosa caused by compound heterozygous mutations in CRB1 (NM_001257965): c.1369C>T (p.R457X) and c.2027C>T (p.T676M). These cells were transfected with four inducible plasmids encoding human OCT4, SOX2, KLF4 or C-MYC together. Transfected cells were induced to form pluripotent stem cells (iPSCs) expressing the pluripotent stem cell markers SOX2, OCT4, KLF4, SSEA4 and NANOG and presenting a normal karyotype. These cells could be differentiated into three germ layers as well as retinal organoids, and thus provide a valuable cellular model for the study of development of major retina diseases. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1873-5061 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1873506120301197; https://doaj.org/toc/1873-5061 |
| DOI: |
10.1016/j.scr.2020.101817 |
| URL الوصول: |
https://doaj.org/article/bd39e9950e5f49eb8a6ab28caea094fe |
| رقم الانضمام: |
edsdoj.bd39e9950e5f49eb8a6ab28caea094fe |
| قاعدة البيانات: |
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