التفاصيل البيبلوغرافية
| العنوان: |
High-throughput process development from gene cloning to protein production |
| المؤلفون: |
Manman Sun, Alex Xiong Gao, Xiuxia Liu, Yankun Yang, Rodrigo Ledesma-Amaro, Zhonghu Bai |
| المصدر: |
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-13 (2023) |
| بيانات النشر: |
BMC, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
Recombinant protein, Process development, High-throughput technology, High-throughput culture, Microbiology, QR1-502 |
| الوصف: |
Abstract In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In addition, with the expansion of protein sequences in databases, the range of possible enzymes of interest for a given application is also increasing. Faced with peer competition, budgetary, and time constraints, companies and laboratories must find ways to develop a robust manufacturing process for recombinant protein production. In this review, we explore high-throughput technologies for recombinant protein expression and present a holistic high-throughput process development strategy that spans from genes to proteins. We discuss the challenges that come with this task, the limitations of previous studies, and future research directions. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1475-2859 |
| Relation: |
https://doaj.org/toc/1475-2859 |
| DOI: |
10.1186/s12934-023-02184-1 |
| URL الوصول: |
https://doaj.org/article/bd19bfc13ff7467f87e0ba5f8b3da5a8 |
| رقم الانضمام: |
edsdoj.bd19bfc13ff7467f87e0ba5f8b3da5a8 |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder