Academic Journal
Molecular epidemiology of Bordetella pertussis in the Philippines in 2012–2014
| العنوان: | Molecular epidemiology of Bordetella pertussis in the Philippines in 2012–2014 |
|---|---|
| المؤلفون: | Salvacion Rosario L. Galit, Nao Otsuka, Yuki Furuse, Daryl Joy V. Almonia, Lydia T. Sombrero, Rosario Z. Capeding, Socorro P. Lupisan, Mariko Saito, Hitoshi Oshitani, Yukihiro Hiramatsu, Keigo Shibayama, Kazunari Kamachi |
| المصدر: | International Journal of Infectious Diseases, Vol 35, Iss C, Pp 24-26 (2015) |
| بيانات النشر: | Elsevier, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Bordetella pertussis, Philippines, Genotype, Multilocus variable-number tandem repeat analysis, Virulence-associated allelic gene, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Objectives: The present study was designed to determine the genotypes of circulating Bordetella pertussis in the Philippines by direct molecular typing of clinical specimens. Methods: Nasopharyngeal swabs (NPSs) were collected from 50 children hospitalized with pertussis in three hospitals during 2012–2014. Multilocus variable-number tandem repeat analysis (MLVA) was performed on the DNA extracts from NPSs. B. pertussis virulence-associated allelic genes (ptxA, prn, and fim3) and the pertussis toxin promoter, ptxP, were also investigated by DNA sequence-based typing. Results: Twenty-six DNA extracts yielded a complete MLVA profile, which were sorted into 10 MLVA types. MLVA type 34 (MT34), which is rare in Australia, Europe, Japan, and the USA, was the predominant strain (50%). Seven MTs (MT29, MT32, MT33, and MT283–286, total 42%) were single-locus variants of MT34, while two (MT141 and MT287, total 8%) were double-locus variants of MT34. All MTs had the combination of virulence-associated allelic genes, ptxP1–ptxA1–prn1–fim3A. Conclusions: The B. pertussis population in the Philippines comprises genetically related strains. These strains are markedly different from those found in patients from other countries where acellular pertussis vaccines are used. The differences in vaccine types between these other countries and the Philippines, where the whole-cell vaccine is still used, may select for distinct populations of B. pertussis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1201-9712 1878-3511 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1201971215000879; https://doaj.org/toc/1201-9712; https://doaj.org/toc/1878-3511 |
| DOI: | 10.1016/j.ijid.2015.04.001 |
| URL الوصول: | https://doaj.org/article/bcf2996e50ca45c5848ffede3bfaf351 |
| رقم الانضمام: | edsdoj.bcf2996e50ca45c5848ffede3bfaf351 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12019712 18783511 |
|---|---|
| DOI: | 10.1016/j.ijid.2015.04.001 |