Academic Journal
Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius)
| العنوان: | Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius) |
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| المؤلفون: | El Tigani Ahmed El Tigani-Asil, Ghada El Derdiri Abdelwahab, El Hadi Ahmed Mohamed Abdu, Abdelnasir Mohammed Adam Terab, Nasareldien Altaib Hussein Khalil, Zhaya Jaber Mohammed Al Marri, Mohd Farouk Yuosf, Asma Abdi Mohamed Shah, Abdelmalik Ibrahim Khalafalla, Hassan Zackaria Ali Ishag |
| المصدر: | Veterinary World, Vol 16, Iss 6, Pp 1277-1283 (2023) |
| بيانات النشر: | Veterinary World, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Animal culture LCC:Veterinary medicine |
| مصطلحات موضوعية: | acid-fast bacteria, dromedary camel, mycobacterium, paratuberculosis, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| الوصف: | Background and Aim: Paratuberculosis (PTB) or John’s disease is a chronic disease of ruminants impeding the reproduction and productivity of the livestock sector worldwide. Since there is a lack of pathological studies explaining the nature and development of the disease in camels, this study aimed to highlight the anatomopathological changes of PTB in camels, which may help in verifying and validating some diagnostic tests used to detect the etiology of the disease in camel tissues. Materials and Methods: In August 2017, at Alselaa border’s Veterinary Clinic of Al Dhafra Region, Western Abu Dhabi, UAE, one imported culled she-camel of 2 years old was subjected to clinical, microscopic, and anatomopathological investigations along with real-time quantitative polymerase chain reaction (q-PCR) to confirm the infection and correlate between clinical signs and pathological lesions of the PTB in dromedary camels. Results: Clinically, typical clinical signs compliant with the pathognomonic gross and histologic lesions of PTB were seen in naturally infected dromedary camel. As presumptive diagnosis microscopically, acid-fast coccobacillus bacterium clumps were demonstrated in direct fecal smears as well as in scraped mucosal and crushed mesenteric lymph node films, and in histopathological sections prepared from a necropsied animal and stained by Ziehl-Neelsen stain. Free and intracellular acid-fast clump phagosomes were further confirmed as Mycobacterium avium subsp. paratuberculosis by q-PCR. Conclusion: Clinical signs and pathological lesions of paratuberculosis in a dromedary camel were found to be similar to those of the other susceptible hosts. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0972-8988 2231-0916 |
| Relation: | https://www.veterinaryworld.org/Vol.16/June-2023/13.pdf; https://doaj.org/toc/0972-8988; https://doaj.org/toc/2231-0916 |
| DOI: | 10.14202/vetworld.2023.1277-1283 |
| URL الوصول: | https://doaj.org/article/bce4a8be398e4f039e110b20408443d2 |
| رقم الانضمام: | edsdoj.bce4a8be398e4f039e110b20408443d2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 09728988 22310916 |
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| DOI: | 10.14202/vetworld.2023.1277-1283 |