Academic Journal
The epithelial-mesenchymal transition induced by transcription factor LEF-1 is independent of β-catenin
| العنوان: | The epithelial-mesenchymal transition induced by transcription factor LEF-1 is independent of β-catenin |
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| المؤلفون: | Wakako Kobayashi, Masayuki Ozawa |
| المصدر: | Biochemistry and Biophysics Reports, Vol 15, Iss , Pp 13-18 (2018) |
| بيانات النشر: | Elsevier, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biology (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Biology (General), QH301-705.5, Biochemistry, QD415-436 |
| الوصف: | Transcription factor lymphoid-enhancer–binding factor 1 (LEF-1) is a key molecule in the Wnt/β-catenin signaling pathway. Slug is one of the Wnt/β-catenin target genes and can induce epithelial–mesenchymal transition (EMT). Previously, we have shown that not only wild-type LEF-1 but also LEF-1 lacking the amino-terminal β-catenin–binding region can induce EMT, suggesting that LEF-1 acts independently of β-catenin. Because it has been reported that LEF-1 interacts with β-catenin outside the amino-terminal domain, namely, in the middle part of the molecule, the possible participation of β-catenin has not been formally ruled out. To determine the involvement of β-catenin in the LEF-1–induced EMT, we produced MDCK cells with a deletion of the β-catenin gene and then expressed LEF-1 in the cells. We found that LEF-1 induced EMT in those cells. In the absence of β-catenin, γ-catenin has been shown to take over the role of β-catenin. To examine this possibility, we first established MDCK cells with a double knockout of β-catenin and γ-catenin genes and then expressed LEF-1 in these cells. We found that LEF-1 can induce EMT in these cells; therefore, we conclude that neither β-catenin nor γ-catenin expression is necessary for the LEF-1–mediated induction of EMT. Keywords: EMT, LEF-1, β-catenin, CRISPR/Cas9, Knockout |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-5808 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405580818300918; https://doaj.org/toc/2405-5808 |
| DOI: | 10.1016/j.bbrep.2018.06.003 |
| URL الوصول: | https://doaj.org/article/bc3c0006cb9a448a847009db409db025 |
| رقم الانضمام: | edsdoj.bc3c0006cb9a448a847009db409db025 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24055808 |
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| DOI: | 10.1016/j.bbrep.2018.06.003 |