التفاصيل البيبلوغرافية
| العنوان: |
Wireless Biosensor System for Real-Time L-Lactic Acid Monitoring in Fish |
| المؤلفون: |
Hideaki Endo, Huifeng Ren, Kengo Hatanaka, Mai Takase, Kyoko Hibi |
| المصدر: |
Sensors, Vol 12, Iss 5, Pp 6269-6281 (2012) |
| بيانات النشر: |
MDPI AG, 2012. |
| سنة النشر: |
2012 |
| المجموعة: |
LCC:Chemical technology |
| مصطلحات موضوعية: |
L-lactic acid, fish, wireless, monitoring, interstitial fluid, Chemical technology, TP1-1185 |
| الوصف: |
We have developed a wireless biosensor system to continuously monitor L-lactic acid concentrations in fish. The blood L-lactic acid level of fish is a barometer of stress. The biosensor comprised Pt-Ir wire (φ0.178 mm) as the working electrode and Ag/AgCl paste as the reference electrode. Lactate oxidase was immobilized on the working electrode using glutaraldehyde. The sensor calibration was linear and good correlated with L-lactic acid levels (R = 0.9959) in the range of 0.04 to 6.0 mg·dL−1. We used the eyeball interstitial sclera fluid (EISF) as the site of sensor implantation. The blood L-lactic acid levels correlated closely with the EISF L-lactic acid levels in the range of 3 to 13 mg·dL−1 (R = 0.8173, n = 26). Wireless monitoring of L-lactic acid was performed using the sensor system in free-swimming fish in an aquarium. The sensor response was stable for over 60 h. Thus, our biosensor provided a rapid and convenient method for real-time monitoring of L-lactic acid levels in fish. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1424-8220 |
| Relation: |
http://www.mdpi.com/1424-8220/12/5/6269; https://doaj.org/toc/1424-8220 |
| DOI: |
10.3390/s120506269 |
| URL الوصول: |
https://doaj.org/article/bbe3049c8ead4c19b13108b984c471b2 |
| رقم الانضمام: |
edsdoj.bbe3049c8ead4c19b13108b984c471b2 |
| قاعدة البيانات: |
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