Academic Journal
Increase of complex I and reduction of complex II mitochondrial activity are possible adaptive effects provoked by fluoride exposure
| العنوان: | Increase of complex I and reduction of complex II mitochondrial activity are possible adaptive effects provoked by fluoride exposure |
|---|---|
| المؤلفون: | Heloisa Aparecida Barbosa Silva Pereira, Tamara Teodoro Araújo, Aline Dionizio, Juliana Sanches Trevizol, Fabrício Soares Pereira, Flávia Godoy Iano, Valdecir Faria Ximenes, Marília Afonso Rabelo Buzalaf |
| المصدر: | Heliyon, Vol 7, Iss 1, Pp e06028- (2021) |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Liver, Fluoride, Oxidative stress, Mitochondria, Mitochondrial activity, Chronic exposure, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | Fluoride (F) can induce changes in the expression of several liver proteins, most of them localized in the mitochondria and its effect is dose- and time-dependent. This study analyzed the effect of distinct F concentrations and exposure periods on the mitochondrial activity of complex I-III and II-III in the liver. Thirty-six 21-day-old male Wistar rats were divided into 2 groups (n = 18) according to the duration of the treatment (20 or 60 days). They were subdivided into 3 subgroups (n = 6) according to the concentration of F (0 mg/L, 15 mg/L or 50 mg/L). After the experimental periods, the animals were anesthetized, liver mitochondria were isolated and stored for activity analyses. The determination of complexes II-III and I-III was based on the reduction of cytochrome c3+ to cytochrome c2+ performed spectrophotometrically. Bioinformatics analyses were performed using data from a previous study (Pereira et al., 2018). The mitochondrial complex I-III was significantly activated in the groups treated with 50 mgF/L for 20 days and 15 mgF/L for 60 days. The complex II-III was significantly reduced in the group treated with the higher F dose for 60 days. The networks indicated more changes in mitochondrial proteins in the group treated with the higher dose for 20 days; the reduction is probably linked to the activation of the complex I-III. The reduction in the complex II-III upon exposure to the higher F dose in the long term might be part of an adaptative mechanism of the body to counteract the deleterious effects of this ion on the energy metabolism. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S240584402100133X; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2021.e06028 |
| URL الوصول: | https://doaj.org/article/ebb07c41b6f342318248186fc6255bdb |
| رقم الانضمام: | edsdoj.bb07c41b6f342318248186fc6255bdb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
|---|---|
| DOI: | 10.1016/j.heliyon.2021.e06028 |