Academic Journal
Analytical and clinical evaluation of Sysmex UF1000i for automated screening of cerebrospinal fluids
| العنوان: | Analytical and clinical evaluation of Sysmex UF1000i for automated screening of cerebrospinal fluids |
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| المؤلفون: | Buoro Sabrina, Ottomano Cosimo, Appassiti Esposito Sara, Gherardi Patrizia, Grazia Alessio Maria, Crippa Alberto, Farina Claudio, Raglio Annibale, Lippi Giuseppe |
| المصدر: | Journal of Medical Biochemistry, Vol 33, Iss 2, Pp 191-196 (2014) |
| بيانات النشر: | Society of Medical Biochemists of Serbia, Belgrade, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Biochemistry |
| مصطلحات موضوعية: | cerebrospinal fluid, meningitis, automation, cytofluorimeter, Biochemistry, QD415-436 |
| الوصف: | Background: We evaluated the performance of Sysmex UF1000i for cell counting and differential cell count, as well as for assessment of bacteria load in cerebrospinal fluid (CSF), as a potential approach for the rapid screening of meningitis or bacterial encephalitis. Methods: We analyzed 77 consecutive CSF samples, 34 of which (44%) displayed leukocyte count >5 white blood cell (WBC)/μL with optical microscopy. Results on the UF-1000i were compared with those obtained by microscopic analysis. Imprecision was evaluated by testing three CSF samples with leukocyte values between 3.5 and 28.8 WBC/μL in 10 replicates. Carry-over was evaluated with the Broughton formula on three CSF pools with leukocyte counts between 93.5 and 132.5 WBC/μL. Linearity was assessed according to CLSI document EP6-A. In the presence of bacteria, identification and antibiogram were performed with Vitex (Biomerieux), except for Neisserie meningitidis (ApiNH, Biomerieux). Sensitivity tests were performed with Vitex and disc diffusion. Results: Optimal correlation was found between UF-1000i and optical microscopy, displaying Pearson's correlation of 0.99 and mean bias of -3.5 WBC/μL (95% CI, from -7.0 to 0.0 WBC/μL). Imprecision varied between 12 and 16%. Linearity was excellent, 4-278 WBC/μL. Carry-over was negligible. ROC analysis yielded AUC of 0.99 for both WBC and bacterial counts. The agreement at threshold >4 WBC/μL was 0.91, with sensitivity and specificity of 1.00 and 0.84. At ≤19 bacteria/μL cut-off, accuracy was 0.98, sensitivity 1.00 and specificity 0.97. Conclusions: According to these results, CSF screening with UF-1000i seems a reliable approach in terms of instrument performance, turnaround time and overall laboratory efficiency. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1452-8258 1452-8266 |
| Relation: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2014/1452-82581402191B.pdf; https://doaj.org/toc/1452-8258; https://doaj.org/toc/1452-8266 |
| URL الوصول: | https://doaj.org/article/ba9df8bafbad45719b77733b467b800b |
| رقم الانضمام: | edsdoj.ba9df8bafbad45719b77733b467b800b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14528258 14528266 |
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