piggyBac Insertional Mutagenesis Screen Identifies a Role for Nuclear RHOA in Human ES Cell Differentiation

التفاصيل البيبلوغرافية
العنوان:	piggyBac Insertional Mutagenesis Screen Identifies a Role for Nuclear RHOA in Human ES Cell Differentiation
المؤلفون:	Sophia Gayle, Yukun Pan, Sean Landrette, Tian Xu
المصدر:	Stem Cell Reports, Vol 4, Iss 5, Pp 926-938 (2015)
بيانات النشر:	Elsevier, 2015.
سنة النشر:	2015
المجموعة:	LCC:Medicine (General) LCC:Biology (General)
مصطلحات موضوعية:	Medicine (General), R5-920, Biology (General), QH301-705.5
الوصف:	The mechanisms regulating human embryonic stem (ES) cell self-renewal and differentiation are not well defined in part due to the lack of tools for forward genetic analysis. We present a piggyBac transposon gain of function screen in human ES cells that identifies DENND2C, which genetically cooperates with NANOG to maintain self-renewal in the presence of retinoic acid. We show that DENND2C negatively regulates RHOA activity, which cooperates with NANOG to block differentiation. It has been recently shown that RHOA exists in the nucleus and is activated by DNA damage; however, its nuclear function remains unknown. We discovered that RHOA associates with DNA and that DENND2C affects nuclear RHOA localization, activity, and DNA association. Our study illustrates the power of piggyBac as a cost-effective, efficient, and easy to use tool for forward genetic screens in human ES cells and provides insight into the role of RHOA in the nucleus.
نوع الوثيقة:	article
وصف الملف:	electronic resource
اللغة:	English
تدمد:	2213-6711
Relation:	http://www.sciencedirect.com/science/article/pii/S221367111500079X; https://doaj.org/toc/2213-6711
DOI:	10.1016/j.stemcr.2015.03.001
URL الوصول:	https://doaj.org/article/ba830b13aded49ecba9052fa0406bd39
رقم الانضمام:	edsdoj.ba830b13aded49ecba9052fa0406bd39
قاعدة البيانات:	Directory of Open Access Journals

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الوصف
تدمد:	22136711
DOI:	10.1016/j.stemcr.2015.03.001