التفاصيل البيبلوغرافية
| العنوان: |
Salmonella Typhimurium with Eight Tandem Copies of blaNDM-1 on a HI2 Plasmid |
| المؤلفون: |
Huijuan Song, Siyu Zou, Yi Huang, Cui Jian, Weiyong Liu, Lei Tian, Lu Gong, Zhongju Chen, Ziyong Sun, Yue Wang |
| المصدر: |
Microorganisms, Vol 12, Iss 1, p 20 (2023) |
| بيانات النشر: |
MDPI AG, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
NDM-1, Salmonella Typhimurium, IncHI2, whole-genome sequencing, PacBio HiFi sequencing, tandem copies, Biology (General), QH301-705.5 |
| الوصف: |
Carbapenem-resistant Salmonella has recently aroused increasing attention. In this study, a total of four sequence type 36 Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) isolates were consecutively isolated from an 11-month-old female patient with a gastrointestinal infection, of which one was sensitive to carbapenems and three were resistant to carbapenems. Via antibiotic susceptibility testing, a carbapenemases screening test, plasmid conjugation experiments, Illumina short-reads, and PacBio HiFi sequencing, we found that all four S. Typhimurium isolates contained a blaCTX-M-14-positive IncI1 plasmid. One carbapenem-sensitive S. Typhimurium isolate then obtained an IncHI2 plasmid carrying blaNDM-1 and an IncP plasmid without any resistance genes during the disease progression. The blaNDM-1 gene was located on a new 30 kb multiple drug resistance region, which is flanked by IS26 and TnAs2, respectively. In addition, the ST_F0903R isolate contained eight tandem copies of the ISCR1 unit (ISCR1-dsbD-trpF-ble-blaNDM-1-ISAba125Δ1), but an increase in MICs to carbapenems was not observed. Our work further provided evidence of the rapid spread and amplification of blaNDM-1 through plasmid. Prompting the recognition of carbapenem-resistant Enterobacterales and the initiation of appropriate infection control measures are essential to avoid the spread of these organisms. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2076-2607 |
| Relation: |
https://www.mdpi.com/2076-2607/12/1/20; https://doaj.org/toc/2076-2607 |
| DOI: |
10.3390/microorganisms12010020 |
| URL الوصول: |
https://doaj.org/article/ba3aa9529f1b4c6ca7f477d7e0ea0f7e |
| رقم الانضمام: |
edsdoj.ba3aa9529f1b4c6ca7f477d7e0ea0f7e |
| قاعدة البيانات: |
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