التفاصيل البيبلوغرافية
| العنوان: |
Detection of coronavirus-2 by real-time reverse transcription polymerase chain reaction in conjunctival swabs from patients with severe form of Coronavirus disease 2019 in São Paulo, Brazil |
| المؤلفون: |
Mariana Akemi Matsura Misawa, Tatiana Tanaka, Tomás Minelli, Pedro Gomes Oliveira Braga, Juliana Mika Kato, Michele Soares Gomes Gouvêa, João Renato Rebello Pinho, Joyce Hisae Yamamoto |
| المصدر: |
Clinics, Vol 76 (2021) |
| بيانات النشر: |
Elsevier España, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Medicine (General) |
| مصطلحات موضوعية: |
COVID-19, Conjunctiva, SARS-CoV-2, Medicine (General), R5-920 |
| الوصف: |
OBJECTIVES: To test conjunctival swabs from patients with laboratory-confirmed severe forms of coronavirus disease 2019 (COVID-19) for the presence of SARS-CoV-2 on real-time reverse-transcription polymerase chain reaction (rRT-PCR). METHODS: Fifty conjunctival swabs were collected from 50 in-patients with laboratory-confirmed severe forms of COVID-19 at the largest teaching hospital and referral center in Brazil (HCFMUSP, São Paulo, SP). The samples were tested for SARS-CoV-2 on rRT-PCR with the primers and probes described in the CDC protocol which amplify the region of the nucleocapsid N gene (2019_nCoV_N1 and 2019_nCoV_N2) of SARS-CoV-2 RNA and compared with naso/oropharyngeal swabs collected within 24 hours of the conjunctival swabs. RESULTS: Five conjunctival samples (10%) tested positive (amplification of the N1 and N2 primer/probe sets) while two conjunctival samples (4%) yielded inconclusive results (amplification of the N1 primer/probe set only). The naso/oropharyngeal swabs were positive for SARS-CoV-2 on rRT-PCR in 34 patients (68%), negative in 14 (28%) and inconclusive in 2 (4%). The 5 patients with positive conjunctival swabs had positive (n=2), negative (n=2) or inconclusive (n=1) naso/oropharyngeal swabs on rRT-PCR. Patients with negative or inconclusive naso/oropharyngeal swabs had the diagnosis of COVID-19 confirmed by previous positive rRT-PCR results or by serology. CONCLUSION: This is the first study to present conjunctival swab rRT-PCR results for SARS-CoV-2 in a Brazilian population. In our sample of 50 patients with severe forms of COVID-19, 10% had positive conjunctival swabs, most of which were correlated with positive naso/oropharyngeal rRT-PCR results. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1980-5322 |
| Relation: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322021000100294&tlng=en; https://doaj.org/toc/1980-5322 |
| DOI: |
10.6061/clinics/2021/e2913 |
| URL الوصول: |
https://doaj.org/article/b982b4e805c941189291ea67730d731b |
| رقم الانضمام: |
edsdoj.b982b4e805c941189291ea67730d731b |
| قاعدة البيانات: |
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