Academic Journal
Elucidating the role of Rhodiola rosea L. in sepsis-induced acute lung injury via network pharmacology: emphasis on inflammatory response, oxidative stress, and the PI3K-AKT pathway
| العنوان: | Elucidating the role of Rhodiola rosea L. in sepsis-induced acute lung injury via network pharmacology: emphasis on inflammatory response, oxidative stress, and the PI3K-AKT pathway |
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| المؤلفون: | Lu Jiang, Dongdong Yang, Zhuoyi Zhang, Liying Xu, Qingyu Jiang, Yixin Tong, Lanzhi Zheng |
| المصدر: | Pharmaceutical Biology, Vol 62, Iss 1, Pp 272-284 (2024) |
| بيانات النشر: | Taylor & Francis Group, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | Rhodiola rosea L, sepsis-induced acute lung injury, pulmonary fibrosis, inflammation, network pharmacology, molecular docking, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Context Sepsis-induced acute lung injury (ALI) is associated with high morbidity and mortality. Rhodiola rosea L. (Crassulaceae) (RR) and its extracts have shown anti-inflammatory, antioxidant, immunomodulatory, and lung-protective effects.Objective This study elucidates the molecular mechanisms of RR against sepsis-induced ALI.Materials and methods The pivotal targets of RR against sepsis-induced ALI and underlying mechanisms were revealed by network pharmacology and molecular docking. Human umbilical vein endothelial cells (HUVECs) were stimulated by 1 μg/mL lipopolysaccharide for 0.5 h and treated with 6.3, 12.5, 25, 50, 100, and 200 μg/mL RR for 24 h. Then, the lipopolysaccharide-stimulated HUVECs were subjected to cell counting kit-8 (CCK-8), enzyme-linked immunosorbent, apoptosis, and Western blot analyses. C57BL/6 mice were divided into sham, model, low-dose (40 mg/kg), mid-dose (80 mg/kg), and high-dose (160 mg/kg) RR groups. The mouse model was constructed through caecal ligation and puncture, and histological, apoptosis, and Western blot analyses were performed for further validation.Results We identified six hub targets (MPO, HRAS, PPARG, FGF2, JUN, and IL6), and the PI3K-AKT pathway was the core pathway. CCK-8 assays showed that RR promoted the viability of the lipopolysaccharide-stimulated HUVECs [median effective dose (ED50) = 18.98 μg/mL]. Furthermore, RR inhibited inflammation, oxidative stress, cell apoptosis, and PI3K-AKT activation in lipopolysaccharide-stimulated HUVECs and ALI mice, which was consistent with the network pharmacology results.Discussion and conclusion This study provides foundational knowledge of the effective components, potential targets, and molecular mechanisms of RR against ALI, which could be critical for developing targeted therapeutic strategies for sepsis-induced ALI. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 13880209 1744-5116 1388-0209 |
| Relation: | https://doaj.org/toc/1388-0209; https://doaj.org/toc/1744-5116 |
| DOI: | 10.1080/13880209.2024.2319117 |
| URL الوصول: | https://doaj.org/article/b91479807e2241588ee60f36146da57a |
| رقم الانضمام: | edsdoj.b91479807e2241588ee60f36146da57a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13880209 17445116 |
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| DOI: | 10.1080/13880209.2024.2319117 |