التفاصيل البيبلوغرافية
| العنوان: |
12-Hydroxyeicosatetraenoic acid participates in angiotensin II afferent arteriolar vasoconstriction by activating L-type calcium channels |
| المؤلفون: |
Shih Shen Yiu, Xueying Zhao, Edward W. Inscho, John D. Imig |
| المصدر: |
Journal of Lipid Research, Vol 44, Iss 12, Pp 2391-2399 (2003) |
| بيانات النشر: |
Elsevier, 2003. |
| سنة النشر: |
2003 |
| المجموعة: |
LCC:Biochemistry |
| مصطلحات موضوعية: |
lipoxygenase, cytosolic calcium, microcirculation, kidney, Biochemistry, QD415-436 |
| الوصف: |
The lipoxygenase (LO) metabolite, 12(S)-hydroxyeicosatetraenoic acid [12(S)-HETE], constricts renal vessels, contributes to the vascular response to angiotensin, and has been implicated in cardiovascular and renal diseases. The current studies were performed to determine if renal microvascular 12(S)-HETE production is stimulated by angiotensin and the contribution of L-type calcium channels to the vasoconstriction elicited by 12(S)-HETE. Angiotensin increased renal microvascular 12(S)-HETE production by 64%, whereas cyclooxygenase metabolite production was not altered. Renal microvessels also expressed platelet-type 12-LO and leukocyte-type 12-LO. In the juxtamedullary preparation, afferent arteriolar diameter averaged 21 ± 1 μm and 12(S)-HETE caused a graded decrease in vessel caliber. The afferent arteriolar response to 12(S)-HETE was abolished during L-type calcium channel inhibition. Renal microvascular smooth muscle cells were studied using fluorescence microscopy. Renal myocyte [Ca2+]i averaged 93 ± 5 nmol/l. The 12(S)-HETE (5 μmol/l) increased myocyte [Ca2+]i to a peak value of 340 ± 55 nmol/l. The peak [Ca2+]i response following exposure to 12(S)-HETE was greatly attenuated in the absence of extracellular Ca2+ or calcium channel blockade.These results demonstrate that renal microvascular 12(S)-HETE production is increased in response to angiotensin, and activation of L-type calcium channels is an important mechanism responsible for the afferent arteriolar vasoconstriction elicited by 12(S)-HETE. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
0022-2275 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S0022227520319581; https://doaj.org/toc/0022-2275 |
| DOI: |
10.1194/jlr.M300183-JLR200 |
| URL الوصول: |
https://doaj.org/article/b89c58322ab244b1a27699bed1cfae4e |
| رقم الانضمام: |
edsdoj.b89c58322ab244b1a27699bed1cfae4e |
| قاعدة البيانات: |
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