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Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns

التفاصيل البيبلوغرافية
العنوان: Endothelial Microvesicles and Soluble Markers of Endothelial Injury in Critically Ill Newborns
المؤلفون: Veronika Vítková, Martin Pánek, Petr Janec, Michaela Šibíková, Václav Vobruba, Martin Haluzík, Jan Živný, Jan Janota
المصدر: Mediators of Inflammation, Vol 2018 (2018)
بيانات النشر: Hindawi Limited, 2018.
سنة النشر: 2018
المجموعة: LCC:Pathology
مصطلحات موضوعية: Pathology, RB1-214
الوصف: Neonatal systemic inflammatory response and multiple organ dysfunction syndrome are the main postnatal insults influencing mortality and morbidity. Critically ill newborns with high predicted mortality are supported by extracorporeal membrane oxygenation (ECMO). Biomarkers of inflammatory response and endothelial injury can be used for early diagnosis and treatment of critical neonatal situations. The aim of our study was to explore plasma proteins and endothelial microvesicles as markers of inflammation and endothelial activation in newborns on ECMO and to compare them with healthy neonates. Thirteen newborns on ECMO and 13 healthy newborns were included in the study. Plasma soluble biomarkers were measured using multiplex immunoassay based on Luminex® xMAP multianalyte profiling platform. The total microvesicle count and plasma level of surface antigen-specific microvesicles were determined by flow cytometry. The plasma concentration of cell-derived microvesicles was measured using annexin-V labeling, and the endothelial origin of microvesicles was determined using lineage-specific antigen labeling of endothelial cell/microvesicle markers (endoglin/CD105, PECAM1/CD31, VEGFR2/CD309, and MadCAM1). Inflammatory markers (procalcitonin, IL-1β, IL-6, and IL-22) were increased in the ECMO group (P
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 0962-9351
1466-1861
Relation: https://doaj.org/toc/0962-9351; https://doaj.org/toc/1466-1861
DOI: 10.1155/2018/1975056
URL الوصول: https://doaj.org/article/b869c593755e4a5d827d97e7c5469ff5
رقم الانضمام: edsdoj.b869c593755e4a5d827d97e7c5469ff5
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:09629351
14661861
DOI:10.1155/2018/1975056