Academic Journal
Modeling monocular form deprivation in rabbits using a simulated-cataract intraocular lens
| العنوان: | Modeling monocular form deprivation in rabbits using a simulated-cataract intraocular lens |
|---|---|
| المؤلفون: | Si-Yi Gu, Li-Ming Xu, Wei-Jie Sun, Li-Li Liang, Lei Lin, Han Zou, Jing-Yuan Xu, Yu Zheng, Yuan-Yuan Li, Yin-Ying Zhao, Ping-Jun Chang, Yun-E Zhao |
| المصدر: | International Journal of Ophthalmology, Vol 17, Iss 12, Pp 2177-2184 (2024) |
| بيانات النشر: | Press of International Journal of Ophthalmology (IJO PRESS), 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Ophthalmology |
| مصطلحات موضوعية: | monocular deprivation, form deprivation, intraocular lens, congenital cataract, amblyopia, Ophthalmology, RE1-994 |
| الوصف: | AIM: To establish an animal model of form deprivation amblyopia based on a simulated cataract intraocular lens (IOLs). METHODS: Poly(dimethyl siloxane)-SiO2 thin films (PSF) with different degrees of opacity as IOL materials were prepared. The light transmission of the PSF-IOL was measured, and its in vitro biosafety was determined by cell counting kit (CCK)-8 assay using the HLEC-B3 cell line and ARPE-19 cell line. Subsequently, the in vivo safety was determined by implanting the PSF-IOL with 10% wt SiO2 into the right eyes of New Zealand white rabbits (PSF-IOL group), and compared with two control groups: contralateral comparison group and normal control (NC) group (Contralateral comparison group: the fellow eye; NC group: a group of binocular normal rabbits without intervention). The flash visual-evoked potentials (F-VEPs) were measured to verify amblyopia. RESULTS: PSFs containing 0, 2%, and 10% wt SiO2 were successfully constructed. The 0 SiO2 PSF was transparent, while the 10% wt SiO2 PSF was completely opaque. It was found that PSF did not induce unwanted cytotoxicity in HLECs and ARPE19 cells in vitro. In vitro, PSF-IOL with 10% wt SiO2 was also non-toxic, and no significant inflammation or structural changes occurred after four weeks of PSF-IOL implantation. Finally, our IOL-simulated congenital cataract rabbit detected by F-VEPs suggested tentative amblyopia. CONCLUSION: A PSF-IOL that mimics cataracts is created. A novel form deprivation model is created by the IOL-simulated congenital cataract rabbit. It can be developed fast and stable and holds great potential for future study. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2222-3959 2227-4898 |
| Relation: | http://ies.ijo.cn/en_publish/2024/12/20241204.pdf; https://doaj.org/toc/2222-3959; https://doaj.org/toc/2227-4898 |
| DOI: | 10.18240/ijo.2024.12.04 |
| URL الوصول: | https://doaj.org/article/acb860101d9649aebdc2d51ec8c64782 |
| رقم الانضمام: | edsdoj.b860101d9649aebdc2d51ec8c64782 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22223959 22274898 |
|---|---|
| DOI: | 10.18240/ijo.2024.12.04 |