التفاصيل البيبلوغرافية
| العنوان: |
Mutation in the Agrobacterium hisI gene enhances transient expression in pepper |
| المؤلفون: |
Dan Liu, Shengnan Zhao, Jubin Wang, Xi Zhang, Yingtian Deng, Feng Li |
| المصدر: |
Horticultural Plant Journal, Vol 10, Iss 3, Pp 809-822 (2024) |
| بيانات النشر: |
KeAi Communications Co., Ltd., 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Plant culture |
| مصطلحات موضوعية: |
Capsicum annuum L., Agrobacterium, Transient transformation efficiency, Mutation, Amino acid, Immune response, Plant culture, SB1-1110 |
| الوصف: |
Agrobacterium-mediated plant transformation is widely used in plant genetic engineering. However, its efficiency is limited by plant immunity against Agrobacterium. Chili pepper (Capsicum annuum L.) is an important vegetable that is recalcitrant to Agrobacterium-mediated transformation. In this work, Agrobacterium was found to induce a strong immune response in pepper, which might be the reason for T-DNA being difficult to express in pepper. An Agrobacterium mutant screen was conducted and a point mutation in the hisI gene was identified due to a weak immune response and enhanced transient expression mediated by this Agrobacterium mutant in pepper leaves. Further genetic analysis revealed that histidine biosynthesis deficiency caused by mutations in many genes of this pathway led to reduced pepper cell death, presumably due to reduced bacterial growth. However, mutation analysis of threonine and tryptophan biosynthesis genes showed that the biosynthesis of different amino acids may play different roles in Agrobacterium growth and stimulating the pepper immune response. The possible application of Agrobacterium amino acid biosynthesis mutations in plant biology was discussed. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2468-0141 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S246801412300167X; https://doaj.org/toc/2468-0141 |
| DOI: |
10.1016/j.hpj.2023.07.003 |
| URL الوصول: |
https://doaj.org/article/b6d8c51850c7467cb0406b3c38d7baea |
| رقم الانضمام: |
edsdoj.b6d8c51850c7467cb0406b3c38d7baea |
| قاعدة البيانات: |
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