Academic Journal

A Step-by-step Protocol for Crossing and Marker-Assisted Breeding of Asian and African Rice Varieties

التفاصيل البيبلوغرافية
العنوان: A Step-by-step Protocol for Crossing and Marker-Assisted Breeding of Asian and African Rice Varieties
المؤلفون: Yugander Arra, Eliza Loo, Devanna, Melissa Stiebner, Wolf Frommer
المصدر: Bio-Protocol, Vol 14, Iss 18 (2024)
بيانات النشر: Bio-protocol LLC, 2024.
سنة النشر: 2024
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: Biology (General), QH301-705.5
الوصف: Improving desirable traits of popular rice varieties is of particular importance for small-scale food producers. Breeding is considered the most ecological and economic approach to improve yield, especially in the context of pest and pathogen-resistant varieties development. Being able to cross rice lines is also a critical step when using current transgene-based genome editing technologies, e.g., to remove transgenes. Moreover, rice breeders have developed accelerated breeding methods, including marker-assisted backcross breeding (MABB) to develop novel rice varieties with in-built resistance to biotic and abiotic stressors, grain, and nutritional quality. MABB is a highly efficient and cost-effective approach in accelerating the improvement of recipient variety by introgressing desirable traits, especially from landrace cultivars and wild rice accessions. Here, we provide a detailed protocol including video instructions for rice crossing and MABB to introgress target trait(s) of interest into the elite rice line. Further, we also highlight tips and tricks to be considered for a successful crossing and MABB.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2331-8325
Relation: https://bio-protocol.org/en/bpdetail?id=5069&type=0; https://doaj.org/toc/2331-8325
DOI: 10.21769/BioProtoc.5069
URL الوصول: https://doaj.org/article/b5d7b13cf4344b9e8757c4f24ceff5a2
رقم الانضمام: edsdoj.b5d7b13cf4344b9e8757c4f24ceff5a2
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:23318325
DOI:10.21769/BioProtoc.5069