Academic Journal

The Desmosomal Cadherin Desmoglein-2 Experiences Mechanical Tension as Demonstrated by a FRET-Based Tension Biosensor Expressed in Living Cells

التفاصيل البيبلوغرافية
العنوان: The Desmosomal Cadherin Desmoglein-2 Experiences Mechanical Tension as Demonstrated by a FRET-Based Tension Biosensor Expressed in Living Cells
المؤلفون: Sindora R. Baddam, Paul T. Arsenovic, Vani Narayanan, Nicole R. Duggan, Carl R. Mayer, Shaston T. Newman, Dahlia A. Abutaleb, Abhinav Mohan, Andrew P. Kowalczyk, Daniel E. Conway
المصدر: Cells, Vol 7, Iss 7, p 66 (2018)
بيانات النشر: MDPI AG, 2018.
سنة النشر: 2018
المجموعة: LCC:Cytology
مصطلحات موضوعية: desmosomes, mechanobiology, cell biophysics, Cytology, QH573-671
الوصف: Cell-cell junctions are critical structures in a number of tissues for mechanically coupling cells together, cell-to-cell signaling, and establishing a barrier. In many tissues, desmosomes are an important component of cell-cell junctions. Loss or impairment of desmosomes presents with clinical phenotypes in the heart and skin as cardiac arrhythmias and skin blistering, respectively. Because heart and skin are tissues that are subject to large mechanical stresses, we hypothesized that desmosomes, similar to adherens junctions, would also experience significant tensile loading. To directly measure mechanical forces across desmosomes, we developed and validated a desmoglein-2 (DSG-2) force sensor, using the existing TSmod Förster resonance energy transfer (FRET) force biosensor. When expressed in human cardiomyocytes, the force sensor reported high tensile loading of DSG-2 during contraction. Additionally, when expressed in Madin-Darby canine kidney (MDCK) epithelial or epidermal (A431) monolayers, the sensor also reported tensile loading. Finally, we observed higher DSG-2 forces in 3D MDCK acini when compared to 2D monolayers. Taken together, our results show that desmosomes experience low levels of mechanical tension in resting cells, with significantly higher forces during active loading.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2073-4409
Relation: http://www.mdpi.com/2073-4409/7/7/66; https://doaj.org/toc/2073-4409
DOI: 10.3390/cells7070066
URL الوصول: https://doaj.org/article/b5a830ecd59548cfa00828a64ab02402
رقم الانضمام: edsdoj.b5a830ecd59548cfa00828a64ab02402
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20734409
DOI:10.3390/cells7070066