التفاصيل البيبلوغرافية
| العنوان: |
Different ethanol exposure durations affect cytochrome P450 2E1-mediated sevoflurane metabolism in rat liver |
| المؤلفون: |
Wei Jiang, Min Zhang, Rui Cao, Xinghao Wang, Youbo Zuo |
| المصدر: |
BMC Anesthesiology, Vol 24, Iss 1, Pp 1-11 (2024) |
| بيانات النشر: |
BMC, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Anesthesiology |
| مصطلحات موضوعية: |
Ethanol, CYP2E1, Sevoflurane, Hexafluoroisopropanol (HFIP), Inhalation anesthesia, Anesthesiology, RD78.3-87.3 |
| الوصف: |
Abstract Background Chronic alcohol users often exhibit an increased minimum alveolar concentration (MAC) of sevoflurane, yet the specific mechanism remains unclear. It has been reported that ethanol exposure can upregulate the protein expression and enzyme activity of cytochrome P450 2E1 (CYP2E1). CYP2E1 is a key enzyme that converts 2–5% of sevoflurane into equimolar amounts of hexafluoroisopropanol (HFIP) and F−. This study aims to explore whether ethanol exposure could alter sevoflurane metabolism through CYP2E1 modulation, potentially explaining the increased MAC observed in alcohol users. Methods Eighty adult male Sprague-Dawley (SD) rats were randomly divided into two groups and received either 50% ethanol (dose: 3 g/kg) or 0.9% saline twice daily by gavage. After 1, 2, 3, and 4 weeks of gavage, ten rats were randomly selected from each group to undergo 1-hour anesthesia with 2.3% sevoflurane. Blood samples were collected after anesthesia to measure the concentration of free HFIP using gas chromatography. Additionally, the left lobe tissue of the liver was collected for the analysis of CYP2E1 protein expression by Western blot and CYP2E1 enzyme activity by colorimetric assay. Correlations between these parameters were analyzed using Pearson’s correlation. Results In the ethanol group, CYP2E1 expression, activity, and the concentration of free HFIP were significantly higher at all time points compared to the control group (P 0.05). Within-group comparisons indicated no significant changes in any of the parameters for the control group (P > 0.05). In the ethanol group, there was no difference in free HFIP concentration between the first and second weeks (P > 0.05), but a significant increase was observed in the third and fourth weeks (P |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1471-2253 |
| Relation: |
https://doaj.org/toc/1471-2253 |
| DOI: |
10.1186/s12871-024-02704-5 |
| URL الوصول: |
https://doaj.org/article/b3db9be5f70a461699a6e891cad7e00a |
| رقم الانضمام: |
edsdoj.b3db9be5f70a461699a6e891cad7e00a |
| قاعدة البيانات: |
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