التفاصيل البيبلوغرافية
| العنوان: |
GenoMine: a CRISPR-Cas9-based kill switch for biocontainment of Pseudomonas putida |
| المؤلفون: |
Enrique Asin-Garcia, Maria Martin-Pascual, Claudia de Buck, Max Allewijn, Alexandra Müller, Vitor A. P. Martins dos Santos |
| المصدر: |
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024) |
| بيانات النشر: |
Frontiers Media S.A., 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Biotechnology |
| مصطلحات موضوعية: |
CRISPR-Cas9-mediated biocontainment, kill switch, riboregulator, genetic circuit, P. putida, Biotechnology, TP248.13-248.65 |
| الوصف: |
Synthetic genetic circuits have revolutionised our capacity to control cell viability by conferring microorganisms with programmable functionalities to limit survival to specific environmental conditions. Here, we present the GenoMine safeguard, a CRISPR-Cas9-based kill switch for the biotechnological workhorse Pseudomonas putida that employs repetitive genomic elements as cleavage targets to unleash a highly genotoxic response. To regulate the system’s activation, we tested various circuit-based mechanisms including the digitalised version of an inducible expression system that operates at the transcriptional level and different options of post-transcriptional riboregulators. All of them were applied not only to directly control Cas9 and its lethal effects, but also to modulate the expression of two of its inhibitors: the AcrIIA4 anti-CRISPR protein and the transcriptional repressor TetR. Either upon direct induction of the endonuclease or under non-induced conditions of its inhibitors, the presence of Cas9 suppressed cell survival which could be exploited beyond biocontainment in situations where further CRISPR genome editing is undesirable. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2296-4185 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fbioe.2024.1426107/full; https://doaj.org/toc/2296-4185 |
| DOI: |
10.3389/fbioe.2024.1426107 |
| URL الوصول: |
https://doaj.org/article/b3281f6fb79b4276a41246913351885d |
| رقم الانضمام: |
edsdoj.b3281f6fb79b4276a41246913351885d |
| قاعدة البيانات: |
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