Academic Journal
Comparing morphology, flow cytometry and molecular genetics in the assessment of minimal residual disease in children with B-acute lymphoblastic leukaemia (B-ALL)
| العنوان: | Comparing morphology, flow cytometry and molecular genetics in the assessment of minimal residual disease in children with B-acute lymphoblastic leukaemia (B-ALL) |
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| المؤلفون: | Candice L. Hendricks, Suvarna Buldeo, Dashini Pillay, Ashendran Naidoo, Rajendra Thejpal, Nadine Rapiti, Beverley Neethling, Yasmin Goga, Hamida van Staaden |
| المصدر: | South African Journal of Oncology, Vol 3, Iss 0, Pp e1-e8 (2019) |
| بيانات النشر: | AOSIS, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | minimal residual disease, b-acute lymphoblastic leukaemia, paediatric, flow cytometry, molecular genetics, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | ackground:Minimal residual disease (MRD) detection has been shown to be the best prognostic factor in B-acute lymphoblastic leukaemia (B-ALL). Multicolour flow cytometry (FCM) and specific molecular aberrations (MOL) are the classic techniques used to assess MRD. The former is faster and less costly. Aim: This study compares morphology and FCM to MOL in detecting MRD. Setting: The study was conducted at Inkosi Albert Luthuli Central Hospital (IALCH). Methods: A retrospective review of children with B-ALL managed at IALCH from January 2013 to January 2018 was conducted. Multicolour flow cytometry was performed using Euroflow® panels. Molecular aberrations looked at common cytogenetic markers. Presentation and post-induction morphology (May–Grunwald Giemsa stain), FCM and MOL data for MRD were analysed. Results: Eleven patients were excluded (6-demised, 5-incomplete records), leaving 64 to be analysed (54% female, median age 5 years). Five post-induction aspirates were unsuitable but the rest (92%) were in morphological remission. At diagnosis and post-induction, 62 (95%) and 61 (94%) children, respectively, had FCM performed. A positive MOL result was found in 39 (60%) patients. MOL turn-around times (TATs) averaged 14 days compared with those of FCM’s average of 3 days. MRD was found in 9 patients (FCM) and 7 patients (MOL). Of these patients, 4 had a good correlation between the two and 2 patients with negative FCM had positive MOL MRD post-induction. Conclusion: Morphology is insensitive in MRD assessment. FCM correlated well with molecular MRD and has the shortest turn-around time. FCM has major benefit in the 40% of patients with negative MOL. It can also be safely used to guide treatment escalation in those patients awaiting molecular results. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2518-8704 2523-0646 |
| Relation: | https://sajo.org.za/index.php/sajo/article/view/76; https://doaj.org/toc/2518-8704; https://doaj.org/toc/2523-0646 |
| DOI: | 10.4102/sajo.v3i0.76 |
| URL الوصول: | https://doaj.org/article/ceb22fc6b85d4fe3901249b488b4a226 |
| رقم الانضمام: | edsdoj.b22fc6b85d4fe3901249b488b4a226 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 25188704 25230646 |
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| DOI: | 10.4102/sajo.v3i0.76 |