التفاصيل البيبلوغرافية
| العنوان: |
Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin |
| المؤلفون: |
Aneela Shah Bano, Abdul Mateen Khattak, Abdul Basit, Mehboob Alam, Syed Tanveer Shah, Naveed Ahmad, Syed Abdul Qadir Gilani, Izhar Ullah, Sumera Anwar, Heba Ibrahim Mohamed |
| المصدر: |
Brazilian Archives of Biology and Technology, Vol 65 (2022) |
| بيانات النشر: |
Instituto de Tecnologia do Paraná (Tecpar), 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Biotechnology |
| مصطلحات موضوعية: |
Antioxidant activity, Growth regulators, Sage, Secondary Metabolites, Biotechnology, TP248.13-248.65 |
| الوصف: |
Abstract: Tissue culture technique is one of the best methods to reproduce salvia plant Therefore, the aim of this research was to enhance the in-vitro callus proliferation and production of secondary metabolites of S. moorcroftiana using different combinations of auxin, cytokinin and melatonin. Initially, callus induction was optimized using indole acetic acid (IAA), 2, 4-dichlorophenoxy acetic acid (2,4-D), and naphthalene acetic acid (NAA) applied at different concentrations in combination with 1 mg L-1 of 6-benzylaminopurine (BAP). The results indicates that earliest days to callus induction (14.67 days) was occurred in the media fortified with 2, 4-D+BAP (2.0+1.0 mgL-1). Whereas the highest callus initiation (100%) was induced on MS medium incorporated with 2,4-D+BAP (1+1mgL-1). Furthermore, maximum fresh weight was obtained when 2,4- D + BAP at the rate of (1+ 1mg L-1) was incorporated and dry weight was attained when 2,4- D + BAP at the rate of (2+1 mg L-1) was added to MS media. The maximum fresh and dry weight was obtained when melatonin at rate of 1.5 mg L-1 was supplemented with MS media including 2,4-D + BAP (1+1mg L-1), moreover the maximum DPPH scavenging activity, total phenolic and flavonoid content was noted when supplemented with melatonin at rate of 1.5 mg L-1. In conclusion, among various concentrations of plant growth regulators, 2,4- D + BAP at the rate of (1+ 1mg L-1) along with 1.5 g L-1 melatonin was the best for callus growth and production of secondary metabolites of S. moorcroftiana. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1678-4324 |
| Relation: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100210&lng=en&tlng=en; http://www.scielo.br/pdf/babt/v65/1516-8913-babt-65-e22210200.pdf; https://doaj.org/toc/1678-4324 |
| DOI: |
10.1590/1678-4324-2022210200 |
| URL الوصول: |
https://doaj.org/article/b12340f5bf9f483bb58114a541dfd990 |
| رقم الانضمام: |
edsdoj.b12340f5bf9f483bb58114a541dfd990 |
| قاعدة البيانات: |
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