التفاصيل البيبلوغرافية
| العنوان: |
Multilayer and MATR3-dependent regulation of mRNAs maintains pluripotency in human induced pluripotent stem cells |
| المؤلفون: |
Daniele Pollini, Rosa Loffredo, Federica Maniscalco, Marina Cardano, Mariachiara Micaelli, Isabelle Bonomo, Nausicaa Valentina Licata, Daniele Peroni, Weronika Tomaszewska, Annalisa Rossi, Valeria Crippa, Erik Dassi, Gabriella Viero, Alessandro Quattrone, Angelo Poletti, Luciano Conti, Alessandro Provenzani |
| المصدر: |
iScience, Vol 24, Iss 3, Pp 102197- (2021) |
| بيانات النشر: |
Elsevier, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Science |
| مصطلحات موضوعية: |
molecular biology, cellular neuroscience, stem cells research, Science |
| الوصف: |
Summary: Matrin3 (MATR3) is a nuclear RNA/DNA-binding protein that plays pleiotropic roles in gene expression regulation by directly stabilizing target RNAs and supporting the activity of transcription factors by modulating chromatin architecture. MATR3 is involved in the differentiation of neural cells, and, here, we elucidate its critical functions in regulating pluripotent circuits in human induced pluripotent stem cells (hiPSCs). MATR3 downregulation affects hiPSCs' differentiation potential by altering key pluripotency regulators' expression levels, including OCT4, NANOG, and LIN28A by pleiotropic mechanisms. MATR3 binds to the OCT4 and YTHDF1 promoters favoring their expression. YTHDF1, in turn, binds the m6A-modified OCT4 mRNA. Furthermore, MATR3 is recruited on ribosomes and controls pluripotency regulating the translation of specific transcripts, including NANOG and LIN28A, by direct binding and favoring their stabilization. These results show that MATR3 orchestrates the pluripotency circuitry by regulating the transcription, translational efficiency, and epitranscriptome of specific transcripts. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004221001656; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2021.102197 |
| URL الوصول: |
https://doaj.org/article/9fcad58d9cd74704a77b3694b80e79df |
| رقم الانضمام: |
edsdoj.9fcad58d9cd74704a77b3694b80e79df |
| قاعدة البيانات: |
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