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Nanoscale observation of PM2.5 incorporated into mammalian cells using scanning electron-assisted dielectric microscope

التفاصيل البيبلوغرافية
العنوان: Nanoscale observation of PM2.5 incorporated into mammalian cells using scanning electron-assisted dielectric microscope
المؤلفون: Tomoko Okada, Tomoaki Iwayama, Shinya Murakami, Masaki Torimura, Toshihiko Ogura
المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
بيانات النشر: Nature Portfolio, 2021.
سنة النشر: 2021
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Abstract PM2.5 has been correlated with risk factors for various diseases and infections. It promotes tissue injury by direct effects of particle components. However, effects of PM2.5 on cells have not been fully investigated. Recently, we developed a novel imaging technology, scanning electron-assisted dielectric-impedance microscopy (SE-ADM), which enables observation of various biological specimens in aqueous solution. In this study, we successfully observed PM2.5 incorporated into living mammalian cells in culture media. Our system directly revealed the process of PM2.5 aggregation in the cells at a nanometre resolution. Further, we found that the PM2.5 aggregates in the intact cells were surrounded by intracellular membrane-like structures of low-density in the SE-ADM images. Moreover, the PM2.5 aggregates were shown by confocal Raman microscopy to be located inside the cells rather than on the cell surface. We expect our method to be applicable to the observation of various nanoparticles inside cells in culture media.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-020-80546-0
URL الوصول: https://doaj.org/article/9f8a3c3a039d47c991d165eb8c68cc22
رقم الانضمام: edsdoj.9f8a3c3a039d47c991d165eb8c68cc22
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-020-80546-0