Academic Journal
Engineered exosomes delivering specific tumor-suppressive RNAi attenuate oral cancer progression
| العنوان: | Engineered exosomes delivering specific tumor-suppressive RNAi attenuate oral cancer progression |
|---|---|
| المؤلفون: | Yutaro Kase, Katsuhiro Uzawa, Sho Wagai, Shusaku Yoshimura, Jun-Ichiro Yamamoto, Yuriko Toeda, Megumi Okubo, Keitaro Eizuka, Toshiaki Ando, Takafumi Nobuchi, Kohei Kawasaki, Tomoaki Saito, Manabu Iyoda, Dai Nakashima, Atsushi Kasamatsu, Hideki Tanzawa |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| بيانات النشر: | Nature Portfolio, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Abstract Exosomes are involved in a wide range of biological processes in human cells. Considerable evidence suggests that engineered exosomes (eExosomes) containing therapeutic agents can attenuate the oncogenic activity of human cancer cells. Despite its biomedical relevance, no information has been available for oral squamous cell carcinoma (OSCC), and therefore the development of specific OSCC-targeting eExosomes (octExosomes) is urgently needed. We demonstrated that exosomes from normal fibroblasts transfected with Epstein–Barr Virus Induced-3 (EBI3) cDNA were electroporated with siRNA of lymphocyte cytoplasmic protein 1 (LCP1), as octExosomes, and a series of experiments were performed to evaluate the loading specificity/effectiveness and their anti-oral cancer cell activities after administration of octExosomes. These experiments revealed that octExosomes were stable, effective for transferring siLCP1 into OSCC cells and LCP1 was downregulated in OSCC cells with octExosomes as compared with their counterparts, leading to a significant tumor-suppressive effect in vitro and in vivo. Here we report the development of a new valuable tool for inhibiting tumor cells. By engineering exosomes, siLCP1 was transferred to specifically suppress oncogenic activity of OSCC cells. Inhibition of other types of human malignant cells merits further study. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-021-85242-1 |
| URL الوصول: | https://doaj.org/article/9ed74c814c914ce78df8087aa1a83150 |
| رقم الانضمام: | edsdoj.9ed74c814c914ce78df8087aa1a83150 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
|---|---|
| DOI: | 10.1038/s41598-021-85242-1 |