Academic Journal
Enhancing cell motility via non-contact capacitively coupled electrostatic field
| العنوان: | Enhancing cell motility via non-contact capacitively coupled electrostatic field |
|---|---|
| المؤلفون: | Isabella Zironi, Tobias Cramer, Alessandro Fuschi, Margherita Cioni, Giada Guerra, Giacomo Giuliani, Maria Calienni, Laura Caramazza, Micaela Liberti, Francesca Apollonio, Daniel Remondini, Gastone Castellani |
| المصدر: | Scientific Reports, Vol 14, Iss 1, Pp 1-18 (2024) |
| بيانات النشر: | Nature Portfolio, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Electric field, Scratch assay, Single-cell movement, Numerical dosimetry, Microdosimetry, Electrical stimulation, Medicine, Science |
| الوصف: | Abstract Cellular motility is essential for making and maintaining multicellular organisms throughout their lifespan. Migrating cells can move either individually or collectively by a crawling movement that links the cytoskeletal activity to the adhesion surface. In vitro stimulation by electric fields can be achieved by direct, capacitive or inductive coupled setups. We tested the effects of electrical stimulation provided by capacitive coupling on glioma cells, using a capacitive-coupled system powered by a potential difference of 35 V between two electrodes placed outside the culture dish. Numerical dosimetry identified two different fields: (i) in the order of 103 V/m at the level of the dielectric substrates, with almost uniform distribution; (ii) in the order of 10−1 V/m at the level of the culture medium, with spatial and material-dependent distribution. The scratch assay and the tracking of single-cell movement showed a boosted motility when crawling occurs on polystyrene surfaces, demonstrating the feasibility of this peculiar exposure system to generate forces capable of influencing cell behavior. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-024-77384-9 |
| URL الوصول: | https://doaj.org/article/de9ed7365f5a4e3b9579cdd404bc37fe |
| رقم الانضمام: | edsdoj.9ed7365f5a4e3b9579cdd404bc37fe |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
|---|---|
| DOI: | 10.1038/s41598-024-77384-9 |