Academic Journal
YeATS - a tool suite for analyzing RNA-seq derived transcriptome identifies a highly transcribed putative extensin in heartwood/sapwood transition zone in black walnut [version 2; referees: 2 approved]
| العنوان: | YeATS - a tool suite for analyzing RNA-seq derived transcriptome identifies a highly transcribed putative extensin in heartwood/sapwood transition zone in black walnut [version 2; referees: 2 approved] |
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| المؤلفون: | Sandeep Chakraborty, Monica Britton, Jill Wegrzyn, Timothy Butterfield, Pedro José Martínez-García, Russell L. Reagan, Basuthkar J. Rao, Charles A. Leslie, Mallikarjuna Aradhaya, David Neale, Keith Woeste, Abhaya M. Dandekar |
| المصدر: | F1000Research, Vol 4 (2015) |
| بيانات النشر: | F1000 Research Ltd, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Agriculture & Biotechnology, Plant Genetics & Gene Expression, Structure: RNA, Structure: Transcription & Translation, Medicine, Science |
| الوصف: | The transcriptome provides a functional footprint of the genome by enumerating the molecular components of cells and tissues. The field of transcript discovery has been revolutionized through high-throughput mRNA sequencing (RNA-seq). Here, we present a methodology that replicates and improves existing methodologies, and implements a workflow for error estimation and correction followed by genome annotation and transcript abundance estimation for RNA-seq derived transcriptome sequences (YeATS - Yet Another Tool Suite for analyzing RNA-seq derived transcriptome). A unique feature of YeATS is the upfront determination of the errors in the sequencing or transcript assembly process by analyzing open reading frames of transcripts. YeATS identifies transcripts that have not been merged, result in broken open reading frames or contain long repeats as erroneous transcripts. We present the YeATS workflow using a representative sample of the transcriptome from the tissue at the heartwood/sapwood transition zone in black walnut. A novel feature of the transcriptome that emerged from our analysis was the identification of a highly abundant transcript that had no known homologous genes (GenBank accession: KT023102). The amino acid composition of the longest open reading frame of this gene classifies this as a putative extensin. Also, we corroborated the transcriptional abundance of proline-rich proteins, dehydrins, senescence-associated proteins, and the DNAJ family of chaperone proteins. Thus, YeATS presents a workflow for analyzing RNA-seq data with several innovative features that differentiate it from existing software. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2046-1402 |
| Relation: | http://f1000research.com/articles/4-155/v2; https://doaj.org/toc/2046-1402 |
| DOI: | 10.12688/f1000research.6617.2 |
| URL الوصول: | https://doaj.org/article/9d15363dcafd430da6b7347db368bdb9 |
| رقم الانضمام: | edsdoj.9d15363dcafd430da6b7347db368bdb9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20461402 |
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| DOI: | 10.12688/f1000research.6617.2 |