Academic Journal
miR-27a inhibits molecular adhesion between monocytes and human umbilical vein endothelial cells; systemic approach
| العنوان: | miR-27a inhibits molecular adhesion between monocytes and human umbilical vein endothelial cells; systemic approach |
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| المؤلفون: | Farhad Shaikhnia, Ghasem Ghasempour, Asghar Mohammadi, Mohammad Shabani, Mohammad Najafi |
| المصدر: | BMC Research Notes, Vol 15, Iss 1, Pp 1-7 (2022) |
| بيانات النشر: | BMC, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Biology (General) LCC:Science (General) |
| مصطلحات موضوعية: | SELP, JAM-B (2), SELE, miR-194, miR-27a, Adhesion, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390 |
| الوصف: | Abstract Objective The endothelial cells overexpress the adhesion molecules in the leukocyte diapedesis pathway, developing vessel subendothelial molecular events. In this study, miR-194 and miR-27a were predicted and investigated on the expression of adhesion molecules in HUVEC cells. The SELE, SELP, and JAM-B adhesion molecules involved in the leukocyte tethering were predicted on the GO-enriched gene network. Following transfection of PEI-miRNA particles into HUVEC cells, the SELE, SELP, and JAM-B gene expression levels were evaluated by real-time qPCR. Furthermore, the monocyte-endothelial adhesion was performed using adhesion assay kit. Results In agreement with the prediction results, the cellular data showed that miR-27a and miR-194 decrease significantly the SELP and JAM-B expression levels in HUVECs (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1756-0500 |
| Relation: | https://doaj.org/toc/1756-0500 |
| DOI: | 10.1186/s13104-022-05920-9 |
| URL الوصول: | https://doaj.org/article/9bf7a916854348d0a21574a3e73d04f5 |
| رقم الانضمام: | edsdoj.9bf7a916854348d0a21574a3e73d04f5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17560500 |
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| DOI: | 10.1186/s13104-022-05920-9 |