Academic Journal
Evaluation of pro-apoptotic activity of Chelidonium majus L. and/or gamma-radiation against ehrlich as carcinoma transplanted in mice
| العنوان: | Evaluation of pro-apoptotic activity of Chelidonium majus L. and/or gamma-radiation against ehrlich as carcinoma transplanted in mice |
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| المؤلفون: | Noura Magdy Thabet, Enas Mahmoud Moustafa, Iman Hesham EL-Khashab |
| المصدر: | Bioscience Journal, Vol 32, Iss 6 (2016) |
| بيانات النشر: | Universidade Federal de Uberlândia, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Agriculture LCC:Biology (General) |
| مصطلحات موضوعية: | caspase-3, bcl-2, timp-1, dna fragmentation, tumor growth, Agriculture, Biology (General), QH301-705.5 |
| الوصف: | This study was to evaluate, the pro-apoptotic activity of ethanolic Chelidonium majus L. (CM) leaf extract and/or gamma-radiation in Ehrlich ascites carcinoma (EAC) bearing mice by measuring tumor volume, apoptotic factors (caspase-3, Bcl-2 and Bax proteins, tumor necrosis factor-alpha (TNF-α), angiogenesis factors (matrix metalloproteinase (MMP-2 and MMP-9); tissue inhibitor of metalloproteinases (TIMP-1), DNA fragmentation, besides histopathological examination. After tumor inoculation, mice received CM (100 mg/body weight/day) on the 10th day and were exposed to whole body γ-radiation (6Gy) on the 17th day. The data obtained reveales that combining CM with γ-radiation exposure induce significant regression in tumor growth, up-regulate Bax, caspase-3, TIMP-1 and inhibit Bcl-2, TNF-α, MMP-(2 and 9). Cytotoxicity is substantiated by increased DNA-fragmentation (comet assay and DNA content) and histological examination. It could be suggested that combining CM with gamma-radiation increased apoptosis of tumor cells which would help to increase the lifespan of mice. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1981-3163 |
| Relation: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/32991; https://doaj.org/toc/1981-3163 |
| DOI: | 10.14393/BJ-v32n1a2016-32991 |
| URL الوصول: | https://doaj.org/article/9be5fd1d0fe04f2586ead67d14ba27b4 |
| رقم الانضمام: | edsdoj.9be5fd1d0fe04f2586ead67d14ba27b4 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 19813163 |
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| DOI: | 10.14393/BJ-v32n1a2016-32991 |