التفاصيل البيبلوغرافية
| العنوان: |
The distinct fate of smooth and rough Mycobacterium abscessus variants inside macrophages |
| المؤلفون: |
Anne-Laure Roux, Albertus Viljoen, Aïcha Bah, Roxane Simeone, Audrey Bernut, Laura Laencina, Therese Deramaudt, Martin Rottman, Jean-Louis Gaillard, Laleh Majlessi, Roland Brosch, Fabienne Girard-Misguich, Isabelle Vergne, Chantal de Chastellier, Laurent Kremer, Jean-Louis Herrmann |
| المصدر: |
Open Biology, Vol 6, Iss 11 (2016) |
| بيانات النشر: |
The Royal Society, 2016. |
| سنة النشر: |
2016 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
mycobacterium abscessus, macrophages, phagosome, innate response, rapid-growing mycobacteria, Biology (General), QH301-705.5 |
| الوصف: |
Mycobacterium abscessus is a pathogenic, rapidly growing mycobacterium responsible for pulmonary and cutaneous infections in immunocompetent patients and in patients with Mendelian disorders, such as cystic fibrosis (CF). Mycobacterium abscessus is known to transition from a smooth (S) morphotype with cell surface-associated glycopeptidolipids (GPL) to a rough (R) morphotype lacking GPL. Herein, we show that M. abscessus S and R variants are able to grow inside macrophages and are present in morphologically distinct phagosomes. The S forms are found mostly as single bacteria within phagosomes characterized by a tightly apposed phagosomal membrane and the presence of an electron translucent zone (ETZ) surrounding the bacilli. By contrast, infection with the R form leads to phagosomes often containing more than two bacilli, surrounded by a loose phagosomal membrane and lacking the ETZ. In contrast to the R variant, the S variant is capable of restricting intraphagosomal acidification and induces less apoptosis and autophagy. Importantly, the membrane of phagosomes enclosing the S forms showed signs of alteration, such as breaks or partial degradation. Although not frequently encountered, these events suggest that the S form is capable of provoking phagosome–cytosol communication. In conclusion, M. abscessus S exhibits traits inside macrophages that are reminiscent of slow-growing mycobacterial species. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2046-2441 |
| Relation: |
https://doaj.org/toc/2046-2441 |
| DOI: |
10.1098/rsob.160185 |
| URL الوصول: |
https://doaj.org/article/9b76ac6e7a1349e1944d848d81561f14 |
| رقم الانضمام: |
edsdoj.9b76ac6e7a1349e1944d848d81561f14 |
| قاعدة البيانات: |
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