Academic Journal
Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water
| العنوان: | Production of anti-Cryptosporidium polyclonal antibodies and standardization of direct immunofluorescence for detecting oocysts in water |
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| المؤلفون: | Silvia Cristina Osaki, Adriana Oliveira Costa, Ludmilla Della Coletta Troiano, Ernesto Renato Kruger, Juliana Tracz Pereira, Nelson Luis Mello Fernandes, Márcia Benedita de Oliveira Silva, Vanete Thomaz Soccol |
| المصدر: | Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 5, Pp 587-590 (2011) |
| بيانات النشر: | Sociedade Brasileira de Medicina Tropical (SBMT), 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine |
| مصطلحات موضوعية: | Anticorpos policlonais, Imunofluorescência direta, Oocistos, Água, Arctic medicine. Tropical medicine, RC955-962 |
| الوصف: | INTRODUCTION: The production of anti-Cryptosporidium polyclonal antibodies and its use in direct immunofluorescence assays to determine the presence of Cryptosporidium in water are described in the present work. METHODS: Two rabbits were immunized with soluble and particulate antigens from purified Cryptosporidium oocysts. The sera produced were prepared for immunoglobulin G extraction, which were then purified and conjugated with fluorescein isothiocyanate (FITC). Slides containing known amounts of oocysts were prepared to determine the sensitivity of the technique. To test the specificity, slides containing Giardia duodenalis cysts were prepared. RESULTS: The conjugate was successfully used in water samples experimentally contaminated with Cryptosporidium oocysts, and it was possible to detect up to five oocysts/spot, corresponding to contamination of 250 oocysts/mL. CONCLUSIONS: The three immunizations performed in the rabbits were enough to produce antibodies against Cryptosporidium, the standard direct immunofluorescence assay permitted the detection of five oocysts in 20% of the samples, and no cross-reaction with Giardia duodenalis cysts occurred. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-9849 0037-8682 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000500011&lng=en&tlng=en; https://doaj.org/toc/1678-9849 |
| DOI: | 10.1590/S0037-86822011000500011 |
| URL الوصول: | https://doaj.org/article/9ac2a2077e6b4b5b845a5c8873617e8a |
| رقم الانضمام: | edsdoj.9ac2a2077e6b4b5b845a5c8873617e8a |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16789849 00378682 |
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| DOI: | 10.1590/S0037-86822011000500011 |