Academic Journal
Identification of ALG3 as a potential prognostic biomarker in lung adenocarcinoma
| العنوان: | Identification of ALG3 as a potential prognostic biomarker in lung adenocarcinoma |
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| المؤلفون: | Yinjiao Yuan, BaoCheng Xie, Dongbo Guo, Caixiang Liu, Guanming Jiang, Guowei Lai, Yu Zhang, Xiarong Hu, Zhiming Wu, Ruinian Zheng, Linxuan Huang |
| المصدر: | Heliyon, Vol 9, Iss 7, Pp e18065- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Lung adenocarcinoma, ALG3, Prognostic biomarker, Immune infiltration, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | Background: The abnormal expression of Alpha-1,3-mannosyltransferase (ALG3) has been implicated in tumor promotion. However, the clinical significance of ALG3 in Lung Adenocarcinoma (LUAD) remains poorly understood. Therefore, we aimed to assess the prognostic value of ALG3 and its association with immune infiltrates in LUAD. Methods: The transcriptional expression profiles of ALG3 were obtained from the Cancer Genome Atlas (TCGA), comparing lung adenocarcinoma tissue with normal tissues. To determine the prognostic significance of AGL3, Kaplan-Meier plotter, and Cox regression analysis were employed. Logistic regression was utilized to analyze the association between ALG3 expression and clinical characteristics. Additionally, a receiver operating characteristic (ROC) curve and a nomogram were constructed. To explore the underlying mechanisms, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and gene set enrichment analysis (GSEA) was conducted. The relationship between AGL3A mRNA expression and immune infiltrates was investigated using the tumor immune estimation resource (TIMER) and tumor-immune system interaction database (TISIDB). Furthermore, an in vitro experiment was performed to assess the impact of ALG3 mRNA on lung cancer stemness abilities and examine key signaling pathway proteins. Results: Our results revealed the ALG3 mRNA and protein expression in patients with LUAD was much higher than that in adjacent normal tissues. High expression of ALG3 was significantly associated with N stage (N0, HR = 1.98, P = 0.002), pathological stage (stage I, HR = 2.09, P = 0.003), and the number of pack years ( |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844023052738; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2023.e18065 |
| URL الوصول: | https://doaj.org/article/9a04230c95804683be18fac08ff75f5a |
| رقم الانضمام: | edsdoj.9a04230c95804683be18fac08ff75f5a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2023.e18065 |