Academic Journal
Elevated brain-derived cell-free DNA among patients with first psychotic episode – a proof-of-concept study
| العنوان: | Elevated brain-derived cell-free DNA among patients with first psychotic episode – a proof-of-concept study |
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| المؤلفون: | Asael Lubotzky, Ilana Pelov, Ronen Teplitz, Daniel Neiman, Adama Smadja, Hai Zemmour, Sheina Piyanzin, Bracha-Lea Ochana, Kirsty L Spalding, Benjamin Glaser, Ruth Shemer, Yuval Dor, Yoav Kohn |
| المصدر: | eLife, Vol 11 (2022) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | DNA methylation, cfDNA, psychosis, liquid biopsy, epigenetics, biomarker, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Schizophrenia is a common, severe, and debilitating psychiatric disorder. Despite extensive research there is as yet no biological marker that can aid in its diagnosis and course prediction. This precludes early detection and intervention. Imaging studies suggest brain volume loss around the onset and over the first few years of schizophrenia, and apoptosis has been proposed as the underlying mechanism. Cell-free DNA (cfDNA) fragments are released into the bloodstream following cell death. Tissue-specific methylation patterns allow the identification of the tissue origins of cfDNA. We developed a cocktail of brain-specific DNA methylation markers, and used it to assess the presence of brain-derived cfDNA in the plasma of patients with a first psychotic episode. We detected significantly elevated neuron- (p=0.0013), astrocyte- (p=0.0016), oligodendrocyte- (p=0.0129), and whole brain-derived (p=0.0012) cfDNA in the plasma of patients during their first psychotic episode (n=29), compared with healthy controls (n=31). Increased cfDNA levels were not correlated with psychotropic medications use. Area under the curve (AUC) was 0.77, with 65% sensitivity at 90% specificity in patients with a psychotic episode. Potential interpretations of these findings include increased brain cell death, disruption of the blood-brain barrier, or a defect in clearance of material from dying brain cells. Brain-specific cfDNA methylation markers can potentially assist early detection and monitoring of schizophrenia and thus allow early intervention and adequate therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/76391; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.76391 |
| URL الوصول: | https://doaj.org/article/99c557cbe6234e8c8dd6f08840d16183 |
| رقم الانضمام: | edsdoj.99c557cbe6234e8c8dd6f08840d16183 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.76391 |