Academic Journal
Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation
| العنوان: | Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation |
|---|---|
| المؤلفون: | Mariko Kawai, Yu-Ki Ohmori, Mai Nishino, Masayo Yoshida, Kaori Tabata, Do-Saku Hirota, Ayako Ryu-Mon, Hiromitsu Yamamoto, Junya Sonobe, Yo-Hei Kataoka, Noriko Shiotsu, Mika Ikegame, Hiroki Maruyama, Toshio Yamamoto, Kazuhisa Bessho, Kiyoshi Ohura |
| المصدر: | European Journal of Histochemistry, Vol 61, Iss 2 (2017) |
| بيانات النشر: | PAGEPress Publications, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Cell fate, skeletal muscle, BMP, gene transfer, in vivo electroporation., Biology (General), QH301-705.5 |
| الوصف: | We previously developed a novel method for gene transfer, which combined a non-viral gene expression vector with transcutaneous in vivo electroporation. We applied this method to transfer the bone morphogenetic protein (BMP) gene and induce ectopic bone formation in rat skeletal muscles. At present, it remains unclear which types of cells can differentiate into osteogenic cells after BMP gene transfer by in vivo electroporation. Two types of stem cells in skeletal muscle can differentiate into osteogenic cells: muscle-derived stem cells, and bone marrow-derived stem cells in the blood. In the present study, we transferred the BMP gene into rat skeletal muscles. We then stained tissues for several muscle-derived stem cell markers (e.g., Pax7, M-cadherin), muscle regeneration-related markers (e.g., Myod1, myogenin), and an inflammatory cell marker (CD68) to follow cell differentiation over time. Our results indicate that, in the absence of BMP, the cell population undergoes muscle regeneration, whereas in its presence, it can differentiate into osteogenic cells. Commitment towards either muscle regeneration or induction of ectopic bone formation appears to occur five to seven days after BMP gene transfer. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1121-760X 2038-8306 |
| Relation: | http://ejh.it/index.php/ejh/article/view/2772; https://doaj.org/toc/1121-760X; https://doaj.org/toc/2038-8306 |
| DOI: | 10.4081/ejh.2017.2772 |
| URL الوصول: | https://doaj.org/article/aa97c91d98f44c71b25bcb0733f9bfad |
| رقم الانضمام: | edsdoj.97c91d98f44c71b25bcb0733f9bfad |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1121760X 20388306 |
|---|---|
| DOI: | 10.4081/ejh.2017.2772 |