التفاصيل البيبلوغرافية
| العنوان: |
The effects of the standardized extracts of on steroidogenesis pathways and aromatase activity in H295R human adrenocortical carcinoma cells |
| المؤلفون: |
Mijie Kim, Yong Joo Park, Huiyeon Ahn, Byeonghak Moon, Kyu Hyuck Chung, Seung Min Oh |
| المصدر: |
Environmental Health and Toxicology, Vol 31 (2016) |
| بيانات النشر: |
Korean Society of Environmental Health and Toxicology, 2016. |
| سنة النشر: |
2016 |
| المجموعة: |
LCC:Environmental sciences |
| مصطلحات موضوعية: |
extracts, Aromatase inhibitor, H295R cells, Steroidogenesis, Environmental sciences, GE1-350 |
| الوصف: |
Objectives Aromatase inhibitors that block estrogen synthesis are a proven first-line hormonal therapy for postmenopausal breast cancer. Although it is known that standardized extract of Ginkgo biloba (EGb761) induces anti-carcinogenic effects like the aromatase inhibitors, the effects of EGb761 on steroidogenesis have not been studied yet. Therefore, the effects of EGb761 on steroidogenesis and aromatase activity was studied using a H295R cell model, which was a good in vitro model to predict effects on human adrenal steroidogenesis. Methods Cortisol, aldosterone, testosterone, and 17β-estradiol were evaluated in the H295R cells by competitive enzyme-linked immunospecific assay after exposure to EGb761. Real-time polymerase chain reaction were performed to evaluate effects on critical genes in steroid hormone production, specifically cytochrome P450 (CYP11/ 17/19/21) and the hydroxysteroid dehydrogenases (3β-HSD2 and 17β-HSD1/4). Finally, aromatase activities were measured with a tritiated water-release assay and by western blotting analysis. Results H295R cells exposed to EGb761 (10 and 100 μg/mL) showed a significant decrease in 17β-estradiol and testosterone, but no change in aldosterone or cortisol. Genes (CYP19 and 17β-HSD1) related to the estrogen steroidogenesis were significantly decreased by EGb761. EGb761 treatment of H295R cells resulted in a significant decrease of aromatase activity as measured by the direct and indirect assays. The coding sequence/ Exon PII of CYP19 gene transcript and protein level of CYP19 were significantly decreased by EGb761. Conclusions These results suggest that EGb761 could regulate steroidogenesis-related genes such as CYP19 and 17β-HSD1, and lead to a decrease in 17β-estradiol and testosterone. The present study provides good information on potential therapeutic effects of EGb761 on estrogen dependent breast cancer. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2233-6567 |
| Relation: |
http://www.e-eht.org/upload/pdf/eht-31-e2016010.pdf; https://doaj.org/toc/2233-6567 |
| DOI: |
10.5620/eht.e2016010 |
| URL الوصول: |
https://doaj.org/article/96fa46df48924a1bb3f6de31254ca27e |
| رقم الانضمام: |
edsdoj.96fa46df48924a1bb3f6de31254ca27e |
| قاعدة البيانات: |
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