Academic Journal
Pico-second resolved Förster resonance energy transfer (FRET) differentiates self-assembled biological macromolecules in aqueous medium
| العنوان: | Pico-second resolved Förster resonance energy transfer (FRET) differentiates self-assembled biological macromolecules in aqueous medium |
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| المؤلفون: | Lopamudra Roy, Dipanjan Mukherjee, Soumendra Singh, Amrita Banerjee, Neha Bhattacharyya, Animesh Halder, Priya Singh, Subhadipta Mukhopadhyay, Kallol Bhattacharya, Ranjan Das, Samir Kumar Pal |
| المصدر: | Chemical Physics Impact, Vol 4, Iss , Pp 100081- (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Physics LCC:Chemistry |
| مصطلحات موضوعية: | FRET, Biological organization, Lipid, Protein, Fat, Physics, QC1-999, Chemistry, QD1-999 |
| الوصف: | Major constituents of all living beings are nano-sized molecular species like lipids, proteins, fatty acids, and nucleic acids which are constantly in communication with each other through a complex interwoven molecular network. Understanding a cellular physiological function at the molecular level requires differentiation of the self-assembled biomolecules. Photo physical studies of 8-anilino-1-napthalenesulphonate (ANS) and Nile red (NR) are used to explore differentiation of these biomolecules. An efficient Förster resonance energy transfer (FRET) from ANS to NR causes significant fluorescence quenching of ANS in lipid, whereas it becomes practically insignificant for protein. From the present study, FRET efficiency appears as a marker for differentiating these self-assembled macromolecules. To this end, a novel physical method based on FRET efficiency has been developed to measure the total lipid content of milk. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2667-0224 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2667022422000202; https://doaj.org/toc/2667-0224 |
| DOI: | 10.1016/j.chphi.2022.100081 |
| URL الوصول: | https://doaj.org/article/9603d049eb004d80a471458161c9f670 |
| رقم الانضمام: | edsdoj.9603d049eb004d80a471458161c9f670 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26670224 |
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| DOI: | 10.1016/j.chphi.2022.100081 |