التفاصيل البيبلوغرافية
| العنوان: |
A Surface Plasmon Resonance Plastic Optical Fiber Biosensor for the Detection of Pancreatic Amylase in Surgically-Placed Drain Effluent |
| المؤلفون: |
Laura Pasquardini, Nunzio Cennamo, Giuseppe Malleo, Lia Vanzetti, Luigi Zeni, Deborah Bonamini, Roberto Salvia, Claudio Bassi, Alessandra Maria Bossi |
| المصدر: |
Sensors, Vol 21, Iss 10, p 3443 (2021) |
| بيانات النشر: |
MDPI AG, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Chemical technology |
| مصطلحات موضوعية: |
optical fiber sensors, surface plasmon resonance, plastic optical fibers, antibody, amylase, postoperative pancreatic fistula, Chemical technology, TP1-1185 |
| الوصف: |
Postoperative pancreatic fistula (POPF), the major driver of morbidity and mortality following pancreatectomy, is caused by an abnormal communication between the pancreatic ductal epithelium and another epithelial surface containing pancreas-derived, enzyme-rich fluid. There is a strong correlation between the amylase content in surgically-placed drains early in the postoperative course and the development of POPF. A simple and cheap method to determine the amylase content from the drain effluent has been eagerly advocated. Here, we developed an amylase optical biosensor, based on a surface plasmon resonance (SPR) plastic optical fiber (POF), metallized with a 60 nm layer of gold and interrogated with white light. The sensor was made specific by coupling it with an anti-amylase antibody. Each surface derivatization step was optimized and studied by XPS, contact angle, and fluorescence. The POF-biosensor was tested for its response to amylase in diluted drain effluents. The volume of sample required was 50 µL and the measurement time was 8 min. The POF-biosensor showed selectivity for amylase, a calibration curve log-linear in the range of 0.8–25.8 U/L and a limit of detection (LOD) of ~0.5 U/L. In preliminary tests, the POF-biosensor allowed for the measurement of the amylase content of diluted surgically-placed drain effluents with an accuracy of >92% with respect to the gold standard. The POF-biosensor allows for reliable measurement and could be implemented to allow for a rapid bedside assessment of amylase value in drains following pancreatectomy. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1424-8220 |
| Relation: |
https://www.mdpi.com/1424-8220/21/10/3443; https://doaj.org/toc/1424-8220 |
| DOI: |
10.3390/s21103443 |
| URL الوصول: |
https://doaj.org/article/95f4e8dff7ec4377870d3386e1c26c9c |
| رقم الانضمام: |
edsdoj.95f4e8dff7ec4377870d3386e1c26c9c |
| قاعدة البيانات: |
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