Academic Journal
Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage
| العنوان: | Detection of Fusarium spp. and Trichoderma spp. and antagonism of Trichoderma sp. in soybean under no-tillage |
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| المؤلفون: | Paola Mendes Milanesi, Elena Blume, Marlove Fátima Brião Muniz, Lia Rejane Silveira Reiniger, Zaida Inês Antoniolli, Emanuele Junges, Manoeli Lupatini |
| المصدر: | Semina: Ciências Agrárias, Vol 34, Iss 6Supl1, Pp 3219-3234 (2013) |
| بيانات النشر: | Universidade Estadual de Londrina, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Agriculture (General) |
| مصطلحات موضوعية: | Glycine max (L.) Merril, Fungi, Population, Root rot, Biological control, Agriculture (General), S1-972 |
| الوصف: | This study aimed i) to quantify the occurrence of Fusarium spp. and Trichoderma spp. in rhizospheric soil, with and without symptoms of Sudden Death Syndrome (SDS) in eight soybean genotypes; ii) morphologically identify isolates of Fusarium spp. from roots with SDS; iii) evaluate the antagonism between Trichoderma spp. and Fusarium spp. isolates from rhizospheric soil and roots from with and without SDS, respectively; and iv) characterize through the ITS1-5.8S-ITS2 region of rDNA the isolates of Trichoderma spp. with better performance in the direct confrontation. The sampling of soil and roots was performed in an experimental area located in Cruz Alta, RS, Brazil. In the laboratory, serial dilutions of soil samples, counting of the number of Colony Forming Units (UFCs/g-1 of rhizospheric soil) were performed as well as isolation for identification of isolates of Fusarium spp. and Trichoderma spp. and testing of direct confrontation. There were significant differences between the population of Trichoderma spp. in the rhizosphere of plants with and without symptoms of SDS. For the population of Fusarium spp., significant difference was observed only in the rhizosphere of plants without symptoms of SDS. In diseased roots the following species were identified: F. solani, F. avenaceum, F. graminearum, F. oxysporum and F. verticillioides. In the test of direct confrontation, eight isolates of Trichoderma spp. achieved the best performance in the antagonism to Fusarium spp. and Trichoderma spp. from areas with symptoms of SDS had a higher control efficiency in vitro. These isolates showed high similarity to the species of T. koningii agregate. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Portuguese |
| تدمد: | 1679-0359 1676-546X |
| Relation: | http://www.uel.br/revistas/uel/index.php/semagrarias/editor/submission/12043; https://doaj.org/toc/1676-546X; https://doaj.org/toc/1679-0359 |
| DOI: | 10.5433/1679-0359.2013v34n6Supl1p3219 |
| URL الوصول: | https://doaj.org/article/95a0572c06e645308c4098f81d6eb003 |
| رقم الانضمام: | edsdoj.95a0572c06e645308c4098f81d6eb003 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16790359 1676546X |
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| DOI: | 10.5433/1679-0359.2013v34n6Supl1p3219 |