Academic Journal
ZAP-70 restoration in mice by in vivo thymic electroporation.
| العنوان: | ZAP-70 restoration in mice by in vivo thymic electroporation. |
|---|---|
| المؤلفون: | Magali Irla, Murielle Saade, Adrien Kissenpfennig, Lionel Franz Poulin, Lee Leserman, Patrice N Marche, Evelyne Jouvin-Marche, François Berger, Catherine Nguyen |
| المصدر: | PLoS ONE, Vol 3, Iss 4, p e2059 (2008) |
| بيانات النشر: | Public Library of Science (PLoS), 2008. |
| سنة النشر: | 2008 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Viral and non-viral vectors have been developed for gene therapy, but their use is associated with unresolved problems of efficacy and safety. Efficient and safe methods of DNA delivery need to be found for medical application. Here we report a new monopolar system of non-viral electro-gene transfer into the thymus in vivo that consists of the local application of electrical pulses after the introduction of the DNA. We assessed the proof of concept of this approach by correcting ZAP-70 deficient severe combined immunodeficiency (SCID) in mice. The thymic electro-gene transfer of the pCMV-ZAP-70-IRES-EGFP vector in these mice resulted in rapid T cell differentiation in the thymus with mature lymphocytes detected by three weeks in secondary lymphoid organs. Moreover, this system resulted in the generation of long-term functional T lymphocytes. Peripheral reconstituted T cells displayed a diversified T cell receptor (TCR) repertoire, and were responsive to alloantigens in vivo. This process applied to the thymus could represent a simplified and effective alternative for gene therapy of T cell immunodeficiencies. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18446234/?tool=EBI; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0002059 |
| URL الوصول: | https://doaj.org/article/ddd9455abee3488286c45c5d97866959 |
| رقم الانضمام: | edsdoj.9455abee3488286c45c5d97866959 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
|---|---|
| DOI: | 10.1371/journal.pone.0002059 |