Academic Journal
Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs
| العنوان: | Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs |
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| المؤلفون: | Manjusha Lekshmi, Sanath H. Kumar, Kooloth Valappil Rajendran, Binaya Bhusan Nayak |
| المصدر: | Water Science and Technology, Vol 83, Iss 5, Pp 1103-1107 (2021) |
| بيانات النشر: | IWA Publishing, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Environmental technology. Sanitary engineering |
| مصطلحات موضوعية: | enteric virus, norovirus gii, rt-pcr, seafood, shellfish, Environmental technology. Sanitary engineering, TD1-1066 |
| الوصف: | Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on the RNA-dependent RNA polymerase gene of norovirus genogroup II (NoV GII). Samples of bivalves were processed for the concentration of virus and extraction of RNA, followed by reverse transcription PCR. A total of 50 molluscan shellfish samples were analyzed, of which 16 samples yielded positive amplifications of norovirus nucleic acid. The PCR method described here, involving a single set of primers, is useful for rapid screening of shellfish for NoV GII. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0273-1223 1996-9732 |
| Relation: | http://wst.iwaponline.com/content/83/5/1103; https://doaj.org/toc/0273-1223; https://doaj.org/toc/1996-9732 |
| DOI: | 10.2166/wst.2021.048 |
| URL الوصول: | https://doaj.org/article/d9134125fe9d4facb15729fa757e4c97 |
| رقم الانضمام: | edsdoj.9134125fe9d4facb15729fa757e4c97 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 02731223 19969732 |
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| DOI: | 10.2166/wst.2021.048 |