Academic Journal
Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B
| العنوان: | Striatal Injury with 6-OHDA Transiently Increases Cerebrospinal GFAP and S100B |
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| المؤلفون: | Cristiane Batassini, Núbia Broetto, Lucas Silva Tortorelli, Milene Borsoi, Caroline Zanotto, Fabiana Galland, Tadeu Mello Souza, Marina Concli Leite, Carlos-Alberto Gonçalves |
| المصدر: | Neural Plasticity, Vol 2015 (2015) |
| بيانات النشر: | Hindawi Limited, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Neurosciences. Biological psychiatry. Neuropsychiatry |
| مصطلحات موضوعية: | Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571 |
| الوصف: | Both glial fibrillary acidic protein (GFAP) and S100B have been used as markers of astroglial plasticity, particularly in brain injury; however, they do not necessarily change in the same time frame or direction. Herein, we induced a Parkinson’s disease (PD) model via a 6-OHDA intrastriatal injection in rats and investigated the changes in GFAP and S100B using ELISA in the substantia nigra (SN), striatum, and cerebrospinal fluid on the 1st, 7th, and 21st days following the injection. The model was validated using measurements of rotational behaviour induced by methylphenidate and tyrosine hydroxylase in the dopaminergic pathway. To our knowledge, this is the first measurement of cerebrospinal fluid S100B and GFAP in the 6-OHDA model of PD. Gliosis (based on a GFAP increase) was identified in the striatum, but not in the SN. We identified a transitory increment of cerebrospinal fluid S100B and GFAP on the 1st and 7th days, respectively. This initial change in cerebrospinal fluid S100B was apparently related to the mechanical lesion. However, the 6-OHDA-induced S100B secretion was confirmed in astrocyte cultures. Current data reinforce the idea that glial changes precede neuronal damage in PD; however, these findings also indicate that caution is necessary regarding the interpretation of data in this PD model. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2090-5904 1687-5443 |
| Relation: | https://doaj.org/toc/2090-5904; https://doaj.org/toc/1687-5443 |
| DOI: | 10.1155/2015/387028 |
| URL الوصول: | https://doaj.org/article/912aaef15d7b4f3bafc193344905ec9b |
| رقم الانضمام: | edsdoj.912aaef15d7b4f3bafc193344905ec9b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20905904 16875443 |
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| DOI: | 10.1155/2015/387028 |