التفاصيل البيبلوغرافية
العنوان: |
Quantification of anandamide content in animal cells and tissues: the normalization makes the difference |
المؤلفون: |
Finazzi-Agrò Alessandro, Bari Monica, Maccarrone Mauro |
المصدر: |
Lipids in Health and Disease, Vol 1, Iss 1, p 4 (2002) |
بيانات النشر: |
BMC, 2002. |
سنة النشر: |
2002 |
المجموعة: |
LCC:Nutritional diseases. Deficiency diseases |
مصطلحات موضوعية: |
Nutritional diseases. Deficiency diseases, RC620-627 |
الوصف: |
Abstract Anandamide (N-arachidonoylethanolamine, AEA) is an endogenous lipid that binds to cannabinoid receptors in the central nervous system and in peripheral cells. Quantitative analysis of AEA is generally based on the normalization to the fresh weight of the samples. Here, we show that the normalization procedure of AEA content is such a critical factor, that it might introduce per se significant discrepancies in the quantification of AEA even in the same sample. We suggest that a rapid, accurate and most reliable reference to quantify AEA and congeners from different sources is the protein content, a common parameter to cells and tissues. |
نوع الوثيقة: |
article |
وصف الملف: |
electronic resource |
اللغة: |
English |
تدمد: |
1476-511X |
Relation: |
http://www.Lipidworld.com/content/1/1/4; https://doaj.org/toc/1476-511X |
DOI: |
10.1186/1476-511X-1-4 |
URL الوصول: |
https://doaj.org/article/910791e2a59945ea9f1ae544918af041 |
رقم الانضمام: |
edsdoj.910791e2a59945ea9f1ae544918af041 |
قاعدة البيانات: |
Directory of Open Access Journals |