Academic Journal

Quantification of anandamide content in animal cells and tissues: the normalization makes the difference

التفاصيل البيبلوغرافية
العنوان: Quantification of anandamide content in animal cells and tissues: the normalization makes the difference
المؤلفون: Finazzi-Agrò Alessandro, Bari Monica, Maccarrone Mauro
المصدر: Lipids in Health and Disease, Vol 1, Iss 1, p 4 (2002)
بيانات النشر: BMC, 2002.
سنة النشر: 2002
المجموعة: LCC:Nutritional diseases. Deficiency diseases
مصطلحات موضوعية: Nutritional diseases. Deficiency diseases, RC620-627
الوصف: Abstract Anandamide (N-arachidonoylethanolamine, AEA) is an endogenous lipid that binds to cannabinoid receptors in the central nervous system and in peripheral cells. Quantitative analysis of AEA is generally based on the normalization to the fresh weight of the samples. Here, we show that the normalization procedure of AEA content is such a critical factor, that it might introduce per se significant discrepancies in the quantification of AEA even in the same sample. We suggest that a rapid, accurate and most reliable reference to quantify AEA and congeners from different sources is the protein content, a common parameter to cells and tissues.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1476-511X
Relation: http://www.Lipidworld.com/content/1/1/4; https://doaj.org/toc/1476-511X
DOI: 10.1186/1476-511X-1-4
URL الوصول: https://doaj.org/article/910791e2a59945ea9f1ae544918af041
رقم الانضمام: edsdoj.910791e2a59945ea9f1ae544918af041
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:1476511X
DOI:10.1186/1476-511X-1-4