Academic Journal
Intra-ovarian injection of bone marrow-derived c-Kit+ cells for ovarian rejuvenation in menopausal rats
| العنوان: | Intra-ovarian injection of bone marrow-derived c-Kit+ cells for ovarian rejuvenation in menopausal rats |
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| المؤلفون: | Sepideh Sheshpari, Mahnaz Shahnazi, Shahin Ahmadian, Mohammad Nouri, Mehran Mesgari Abbasi, Rahim Beheshti, Reza Rahbarghazi, Ali Honaramooz, Mahdi Mahdipour |
| المصدر: | BioImpacts, Vol 12, Iss 4, Pp 325-335 (2022) |
| بيانات النشر: | Tabriz University of Medical Sciences, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | premature ovarian failure, bone marrow c-kit+ cells, follicular competence, angiogenesis, fertility, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Introduction: Cell-based therapies with certain cell types are touted as novel and hopeful therapeutic intervention in the clinical setting. Here, we aimed to assess the regenerative potential of c-Kit+ cells in the rejuvenation of ovarian tissue and fertility rate in rat model of premature ovarian failure (POF). Methods: Rats were treated with 160 mg/kg/BW of 4-vinylcyclohexene dioxide for 15 days. Freshly enriched rat bone marrow-derived c-Kit+ (MACS) and c-Kit- cells (4×105 cells/10 µL) were transplanted into the ovaries of treatment and control animals. Prior to transplantation as well as 2, 4, 6, and 8 weeks post-transplantation, randomly-selected rats were euthanized and ovarian tissues were subjected to pathophysiological examinations and real-time PCR analyses. Results: POF status was confirmed by the presence of pathological features and a decreased number of immature and mature follicles compared with the control group (P0.05). Notable reduction of collagen deposition was found in c-Kit-treated rats. Transplantation of c-Kit+ cells also restored the reduced fertility rate (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2228-5660 2228-5652 |
| Relation: | https://bi.tbzmed.ac.ir/PDF/bi-12-325.pdf; https://doaj.org/toc/2228-5660; https://doaj.org/toc/2228-5652 |
| DOI: | 10.34172/bi.2021.23499 |
| URL الوصول: | https://doaj.org/article/906456248b00488c99b03f7e4d493120 |
| رقم الانضمام: | edsdoj.906456248b00488c99b03f7e4d493120 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22285660 22285652 |
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| DOI: | 10.34172/bi.2021.23499 |